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10.1016/j.jcv.2014.06.013

http://scihub22266oqcxt.onion/10.1016/j.jcv.2014.06.013
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suck abstract from ncbi


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pmid24998424      J+Clin+Virol 2014 ; 61 (1): 9-19
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  • Deep Sequencing: Becoming a Critical Tool in Clinical Virology #MMPMID24998424
  • QUIÑONES-MATEU ME; AVILA S; REYES-TERAN G; MARTINEZ MA
  • J Clin Virol 2014[Sep]; 61 (1): 9-19 PMID24998424show ga
  • Population (Sanger) sequencing has been the standard method in basic and clinical DNA sequencing for almost 40 years; however, next-generation (deep) sequencing methodologies are now revolutionizing the field of genomics, and clinical virology is no exception. Deep sequencing is highly efficient, producing an enormous amount of information at low cost in a relatively short period of time. High-throughput sequencing techniques have enabled significant contributions to multiples areas in virology, including virus discovery and metagenomics (viromes), molecular epidemiology, pathogenesis, and studies of how viruses to escape the host immune system and antiviral pressures. In addition, new and more affordable deep sequencing-based assays are now being implemented in clinical laboratories. Here we review the use of the current deep sequencing platforms in virology, focusing on three of the most studied viruses: human immunodeficiency virus (HIV), hepatitis C virus (HCV), and influenza virus.
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