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10.1016/j.bpj.2014.05.027 http://scihub22266oqcxt.onion/10.1016/j.bpj.2014.05.027 C4119266!4119266 !24988341     free     free     free Warning: file_get_contents(https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pubmed&id=24988341 &cmd=llinks): Failed to open stream: HTTP request failed! 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Chromatin dynamics during DNA repair revealed by pair correlation analysis of molecular flow in the nucleus |pdf=|usr=}}{{24988341 }} gab.com Text Chromatin dynamics during DNA repair revealed by pair correlation analysis of molecular flow in the nucleus JO: Biophys J http://www.kidney.de/pget.php?&tw=1&pmid=24988341 #FOAvip Chromatin dynamics modulate DNA repair factor accessibility throughout the DNA damage response. The spatiotemporal scale upon which these dynamics occur render them invisible to live cell imaging. Here we present a believed novel assay to monitor the in vivo structural rearrangements of chromatin during DNA repair. By pair correlation analysis of EGFP molecular flow into chromatin before and after damage, this assay measures millisecond variations in chromatin compaction with submicron resolution. Combined with laser microirradiation we employ this assay to monitor the real-time accessibility of DNA at the damage site. We find from comparison of EGFP molecular flow with a molecule that has an affinity toward double-strand breaks (Ku-EGFP) that DNA damage induces a transient decrease in chromatin compaction at the damage site and an increase in compaction to adjacent regions, which together facilitate DNA repair factor recruitment to the lesion with high spatiotemporal control. Twit Text FOAVip Chromatin dynamics during DNA repair revealed by pair correlation analysis of molecular flow in the nucleus ????Biophys J http://www.kidney.de/pget.php?&tw=1&pmid=24988341 #FOAvip Twit Text # Free Paper on # # # # # LINK http://www.kidney.de/pget.php?&tw=1&pmid=24988341 #FOAvip English Wikipedia <ref>{{Cite pmid|24988341 }}</ref> Chromatin dynamics during DNA repair revealed by pair correlation analysis of molecular flow in the nucleus #MMPMID24988341 Hinde E ; Kong X ; Yokomori K ; Gratton E Biophys J 2014[Jul]; 107 (1 ): 55-65 PMID24988341 show ga Chromatin dynamics modulate DNA repair factor accessibility throughout the DNA damage response. The spatiotemporal scale upon which these dynamics occur render them invisible to live cell imaging. Here we present a believed novel assay to monitor the in vivo structural rearrangements of chromatin during DNA repair. By pair correlation analysis of EGFP molecular flow into chromatin before and after damage, this assay measures millisecond variations in chromatin compaction with submicron resolution. Combined with laser microirradiation we employ this assay to monitor the real-time accessibility of DNA at the damage site. We find from comparison of EGFP molecular flow with a molecule that has an affinity toward double-strand breaks (Ku-EGFP) that DNA damage induces a transient decrease in chromatin compaction at the damage site and an increase in compaction to adjacent regions, which together facilitate DNA repair factor recruitment to the lesion with high spatiotemporal control. |*Recombinational DNA Repair [MESH] |Cell Nucleus/*genetics [MESH] |Chromatin/chemistry/*genetics [MESH] |DNA Breaks, Double-Stranded [MESH] |HeLa Cells [MESH]Chromatin dynamics during DNA repair revealed by pair correlation anal(epmc).pdf DeepDyve Pubget Overpricing