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Knockdown of astrocyte elevated gene-1 (AEG-1) in cervical cancer cells decreases
their invasiveness, epithelial to mesenchymal transition, and chemoresistance
#MMPMID24675891
Liu X
; Wang D
; Liu H
; Feng Y
; Zhu T
; Zhang L
; Zhu B
; Zhang Y
Cell Cycle
2014[]; 13
(11
): 1702-7
PMID24675891
show ga
During cancer development, epithelial-mesenchymal transition (EMT) facilitates
tumor dissemination and metastatic spread, which is characterized by morphologic
changes from epithelial cells to fibroblast-like cells, disassembly of
intercellular junction, and increased cell motility. Overexpression of astrocyte
elevated gene-1(AEG-1) in various cancer cell lines and cancers has been found to
be associated with aggressive tumor behavior. We found that AEG-1 expression was
elevated in low differentiation cervical cancer specimens from patients. However,
little is known about the AEG-1's precise role in invasion and metastasis. Here
we demonstrate that downregulation of AEG-1 by RNAi significantly decreased the
invasion and migration of cervical cancer cells, suggesting that AEG-1
overexpression may enhance cancer cell motility by inducing EMT. Downregulation
of AEG-1 also led to reduced expression of mesenchymal marker vimentin and the
transcription factor Snail but upregulation of epithelial marker E-cadherin in
HeLa cells. In addition, knockdown of AEG-1 decreased colony forming units and
increased sensitivity to cancer drugs in vitro. Taken together, our results
suggest that knockdown of AEG-1 could decrease EMT and chemoresistance in
cervical cancer cells and attenuate their aggressive behavior.
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