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Epidermal growth factor-like domain 7 is a marker of the endothelial lineage and
active angiogenesis
#MMPMID24740971
Bambino K
; Lacko LA
; Hajjar KA
; Stuhlmann H
Genesis
2014[Jul]; 52
(7
): 657-70
PMID24740971
show ga
Epidermal growth factor-like domain 7 (Egfl7) expression in the developing embryo
is largely restricted to sites of mesodermal progenitors of
angioblasts/hemangioblasts and the vascular endothelium. We hypothesize that
Egfl7 marks the endothelial lineage during embryonic development, and can be used
to define the emergence of endothelial progenitor cells, as well as to visualize
newly-forming vasculature in the embryo and during the processes of physiologic
and pathologic angiogenesis in the adult. We have generated a transgenic mouse
strain that expresses enhanced green fluorescent protein (eGFP) under the control
of a minimal Egfl7 regulatory sequence (Egfl7:eGFP). Expression of the transgene
recapitulated that of endogenous Egfl7 at sites of vasculogenesis and
angiogenesis in the allantois, yolk sac, and in the embryo proper. The transgene
was not expressed in the quiescent endothelium of most adult organs. However, the
uterus and ovary, which undergo vascular growth and remodeling throughout the
estrus cycle, expressed high levels of Egfl7:eGFP. Importantly, expression of the
Egfl7:eGFP transgene was induced in adult neovasculature. We also found that
increased Egfl7 expression contributed to pathologic revascularization in the
mouse retina. To our knowledge, this is the first mouse model that enables
monitoring of endothelial cells at sites of active vasculogenesis and
angiogenesis. This model also facilitated the isolation and characterization of
EGFL7(+) endothelial cell populations by fluorescence activated cell sorting
(FACS). Together, our results demonstrate that the Egfl7:eGFP reporter mouse is a
valuable tool that can be used to elucidate the mechanisms by which blood vessels
form during development and under pathologic circumstances.
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