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10.1016/j.molcel.2014.05.008 http://scihub22266oqcxt.onion/10.1016/j.molcel.2014.05.008 C4106038!4106038!24882210     free     free     free Deprecated: Implicit conversion from float 233.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 233.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 233.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 233.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 233.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 233.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534       Mol+Cell 2014 ; 55 (2): 253-63 Nephropedia Template TP {{tp|p=24882210|t=2014. Tracing compartmentalized NADPH metabolism in the cytosol and mitochondria of mammalian cells |pdf=|usr=}}{{24882210}} gab.com Text Tracing compartmentalized NADPH metabolism in the cytosol and mitochondria of mammalian cells JO: Mol Cell http://www.kidney.de/pget.php?&tw=1&pmid=24882210 #FOAvip Eukaryotic cells compartmentalize biochemical processes in different organelles, often relying on metabolic cycles to shuttle reducing equivalents across intracellular membranes. NADPH serves as the electron carrier for the maintenance of redox homeostasis and reductive biosynthesis, with separate cytosolic and mitochondrial pools providing reducing power in each respective location. This cellular organization is critical for numerous functions but complicates analysis of metabolic pathways using available methods. Here we develop an approach to resolve NADP(H)-dependent pathways present within both the cytosol and the mitochondria. By tracing hydrogen in compartmentalized reactions that use NADPH as a cofactor, including the production of 2-hydroxyglutarate by mutant isocitrate dehydrogenase enzymes, we can observe metabolic pathway activity in these distinct cellular compartments. Using this system we determine the direction of serine/glycine interconversion within the mitochondria and cytosol, highlighting the ability of this approach to resolve compartmentalized reactions in intact cells. Twit Text FOAVip Tracing compartmentalized NADPH metabolism in the cytosol and mitochondria of mammalian cells ????Mol Cell http://www.kidney.de/pget.php?&tw=1&pmid=24882210 #FOAvip Twit Text # Free Paper on # # # # # LINK http://www.kidney.de/pget.php?&tw=1&pmid=24882210 #FOAvip English Wikipedia <ref>{{Cite pmid|24882210}}</ref> Tracing compartmentalized NADPH metabolism in the cytosol and mitochondria of mammalian cells #MMPMID24882210 Lewis CA; Parker SJ; Fiske BP; McCloskey D; Gui DY; Green CR; Vokes NI; Feist AM; Heiden MGV; Metallo CM Mol Cell 2014[Jul]; 55 (2): 253-63 PMID24882210show ga Eukaryotic cells compartmentalize biochemical processes in different organelles, often relying on metabolic cycles to shuttle reducing equivalents across intracellular membranes. NADPH serves as the electron carrier for the maintenance of redox homeostasis and reductive biosynthesis, with separate cytosolic and mitochondrial pools providing reducing power in each respective location. This cellular organization is critical for numerous functions but complicates analysis of metabolic pathways using available methods. Here we develop an approach to resolve NADP(H)-dependent pathways present within both the cytosol and the mitochondria. By tracing hydrogen in compartmentalized reactions that use NADPH as a cofactor, including the production of 2-hydroxyglutarate by mutant isocitrate dehydrogenase enzymes, we can observe metabolic pathway activity in these distinct cellular compartments. Using this system we determine the direction of serine/glycine interconversion within the mitochondria and cytosol, highlighting the ability of this approach to resolve compartmentalized reactions in intact cells. äTracing compartmentalized NADPH metabolism in the cytosol and mitochon(epmc).pdf DeepDyve Pubget Overpricing