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10.1039/c4ib00050a http://scihub22266oqcxt.onion/10.1039/c4ib00050a C4098877!4098877!24871928     free     free     free Deprecated: Implicit conversion from float 211.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 211.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 211.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 211.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 211.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 245.2 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 245.2 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534       Integr+Biol+(Camb) 2014 ; 6 (7): 673-84 Nephropedia Template TP {{tp|p=24871928|t=2014. Dynamics and evolution of ?-catenin-dependent Wnt signaling revealed through massively parallel clonogenic screening |pdf=|usr=}}{{24871928}} gab.com Text Dynamics and evolution of -catenin-dependent Wnt signaling revealed through massively parallel clonogenic screening JO: Integr Biol (Camb) http://www.kidney.de/pget.php?&tw=1&pmid=24871928 #FOAvip Wnt/?-catenin signaling is of significant interest due to the roles it plays in regulating development, tissue regeneration and disease. Transcriptional reporters have been widely employed to study Wnt/?-catenin signal transduction in live cells and whole organisms and have been applied to understanding embryonic development, exploring oncogenesis and developing therapeutics. Polyclonal heterogeneity in reporter cell lines has historically been seen as a challenge to be overcome in the development of novel cell lines and reporter-based assays, and monoclonal reporter cell lines are commonly employed to reduce this variability. A375 cell lines infected with a reporter for Wnt/?-catenin signaling were screened over short (< 6) and long (> 25) generational timescales. To characterize phenotypic divergence these time-scales, a microfabricated cell array-based screen was developed enabling characterization of 1,119 clonal colonies in parallel. This screen revealed phenotypic divergence after <6 generations at a similar scale to that observed in monoclonal cell lines cultured for >25 generations. Not only were reporter dynamics observed to diverge widely, but monoclonal cell lines were observed with seemingly opposite signaling phenotypes. Additionally, these observations revealed a generational-dependent trend in Wnt signaling in A375 cells that provide insight into the pathway?s mechanisms of positive feedback and self-inhibition. Twit Text FOAVip Dynamics and evolution of -catenin-dependent Wnt signaling revealed through massively parallel clonogenic screening ????Integr Biol (Camb) http://www.kidney.de/pget.php?&tw=1&pmid=24871928 #FOAvip Twit Text # Free Paper on # # # # # LINK http://www.kidney.de/pget.php?&tw=1&pmid=24871928 #FOAvip English Wikipedia <ref>{{Cite pmid|24871928}}</ref> Dynamics and evolution of ?-catenin-dependent Wnt signaling revealed through massively parallel clonogenic screening #MMPMID24871928 Shah PK; Walker MP; Sims CE; Major MB; Allbritton NL Integr Biol (Camb) 2014[Jul]; 6 (7): 673-84 PMID24871928show ga Wnt/?-catenin signaling is of significant interest due to the roles it plays in regulating development, tissue regeneration and disease. Transcriptional reporters have been widely employed to study Wnt/?-catenin signal transduction in live cells and whole organisms and have been applied to understanding embryonic development, exploring oncogenesis and developing therapeutics. Polyclonal heterogeneity in reporter cell lines has historically been seen as a challenge to be overcome in the development of novel cell lines and reporter-based assays, and monoclonal reporter cell lines are commonly employed to reduce this variability. A375 cell lines infected with a reporter for Wnt/?-catenin signaling were screened over short (< 6) and long (> 25) generational timescales. To characterize phenotypic divergence these time-scales, a microfabricated cell array-based screen was developed enabling characterization of 1,119 clonal colonies in parallel. This screen revealed phenotypic divergence after <6 generations at a similar scale to that observed in monoclonal cell lines cultured for >25 generations. Not only were reporter dynamics observed to diverge widely, but monoclonal cell lines were observed with seemingly opposite signaling phenotypes. Additionally, these observations revealed a generational-dependent trend in Wnt signaling in A375 cells that provide insight into the pathway?s mechanisms of positive feedback and self-inhibition. äDynamics and evolution of ?-catenin-dependent Wnt signaling revealed t(epmc).pdf DeepDyve Pubget Overpricing