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10.1016/j.cell.2014.04.039 http://scihub22266oqcxt.onion/10.1016/j.cell.2014.04.039 C4097317!4097317!24949979     free     free     free Deprecated: Implicit conversion from float 209.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 209.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 209.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 209.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 209.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534       Cell 2014 ; 157 (7): 1724-34 Nephropedia Template TP {{tp|p=24949979|t=2014. High-sensitivity measurements of multiple kinase activities in live single cells |pdf=|usr=}}{{24949979}} gab.com Text High-sensitivity measurements of multiple kinase activities in live single cells JO: Cell http://www.kidney.de/pget.php?&tw=1&pmid=24949979 #FOAvip Increasing evidence has shown that population dynamics are qualitatively different from single cell behaviors. Reporters to probe dynamic, single cell behaviors are desirable, yet relatively scarce. Here we describe an easy-to-implement and generalizable technology to generate reporters of kinase activity for individual cells. Our technology converts phosphorylation into a nucleocytoplasmic shuttling event that can be measured by epifluorescence microscopy. Our reporters reproduce kinase activity for multiple types of kinases, and allow for calculation of active kinase concentrations via a mathematical model. Using this technology, we made several experimental observations that had previously been technically unfeasible, including stimulus-dependent patterns of c-Jun N-Terminal Kinase (JNK) and Nuclear Factor kappa B (NF-?B) activation. We also measured JNK, p38 and ERK activities simultaneously, finding that p38 regulates the peak number, but not the intensity, of ERK fluctuations. Our approach opens the possibility of analyzing a wide range of kinase-mediated processes in individual cells. Twit Text FOAVip High-sensitivity measurements of multiple kinase activities in live single cells ????Cell http://www.kidney.de/pget.php?&tw=1&pmid=24949979 #FOAvip Twit Text # Free Paper on # # # # # LINK http://www.kidney.de/pget.php?&tw=1&pmid=24949979 #FOAvip English Wikipedia <ref>{{Cite pmid|24949979}}</ref> High-sensitivity measurements of multiple kinase activities in live single cells #MMPMID24949979 Regot S; Hughey JJ; Bajar BT; Carrasco S; Covert MW Cell 2014[Jun]; 157 (7): 1724-34 PMID24949979show ga Increasing evidence has shown that population dynamics are qualitatively different from single cell behaviors. Reporters to probe dynamic, single cell behaviors are desirable, yet relatively scarce. Here we describe an easy-to-implement and generalizable technology to generate reporters of kinase activity for individual cells. Our technology converts phosphorylation into a nucleocytoplasmic shuttling event that can be measured by epifluorescence microscopy. Our reporters reproduce kinase activity for multiple types of kinases, and allow for calculation of active kinase concentrations via a mathematical model. Using this technology, we made several experimental observations that had previously been technically unfeasible, including stimulus-dependent patterns of c-Jun N-Terminal Kinase (JNK) and Nuclear Factor kappa B (NF-?B) activation. We also measured JNK, p38 and ERK activities simultaneously, finding that p38 regulates the peak number, but not the intensity, of ERK fluctuations. Our approach opens the possibility of analyzing a wide range of kinase-mediated processes in individual cells. äHigh-sensitivity measurements of multiple kinase activities in live si(epmc).pdf DeepDyve Pubget Overpricing