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Deprecated: Implicit conversion from float 213.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Curr+Opin+Chem+Biol 2014 ; ä (ä): 46-53 Nephropedia Template TP
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Faster Fluorescence Microscopy: Advances in High Speed Biological Imaging #MMPMID24815857
Winter PW; Shroff H
Curr Opin Chem Biol 2014[Jun]; ä (ä): 46-53 PMID24815857show ga
The past decade has seen explosive growth in new high speed imaging methods. These can broadly be classified as either point-scanning (which offer better depth penetration) or parallelized systems (which offer higher speed). We discuss each class generally, and cover specific advances in diffraction-limited microscopes (laser-scanning confocal, spinning-disk, and light-sheet) and super-resolution microscopes (single-molecule imaging, stimulated emission-depletion, and structured illumination). A theme of our review is that there is no free lunch: each technique has strengths and weaknesses, and an advance in speed usually comes at the expense of either spatial resolution or depth penetration.