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10.1111/mmi.12644 http://scihub22266oqcxt.onion/10.1111/mmi.12644 C4095994!4095994!24811454     free     free     free Deprecated: Implicit conversion from float 229.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 229.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 229.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 229.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 229.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 229.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 263.2 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 263.2 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534       Mol+Microbiol 2014 ; 93 (1): 98-112 Nephropedia Template TP {{tp|p=24811454|t=2014. The three major types of CRISPR-Cas systems function independently in CRISPR RNA biogenesis in Streptococcus thermophilus |pdf=|usr=}}{{24811454}} gab.com Text The three major types of CRISPR-Cas systems function independently in CRISPR RNA biogenesis in Streptococcus thermophilus JO: Mol Microbiol http://www.kidney.de/pget.php?&tw=1&pmid=24811454 #FOAvip CRISPR-Cas systems are small RNA-based immune systems that protect prokaryotes from invaders such as viruses and plasmids. We have investigated the features and biogenesis of the CRISPR (cr)RNAs in Streptococcus thermophilus (Sth) strain DGCC7710, which possesses four different CRISPR-Cas systems including representatives from the three major types of CRISPR-Cas systems. Our results indicate that the crRNAs from each CRISPR locus are specifically processed into divergent crRNA species by Cas proteins (and non-coding RNAs) associated with the respective locus. We find that the Csm Type III-A and Cse Type I-E crRNAs are specifically processed by Cas6 and Cse3 (Cas6e), respectively, and retain an 8-nucleotide CRISPR repeat sequence tag 5? of the invader-targeting sequence. The Cse Type I-E crRNAs also retain a 21-nucleotide 3? repeat tag. The crRNAs from the two Csn Type II-A systems in Sth consist of a 5?-truncated targeting sequence and a 3? tag; however these are distinct in size between the two. Moreover, the Csn1 (Cas9) protein associated with one Csn locus functions specifically in the production of crRNAs from that locus. Our findings indicate that multiple CRISPR-Cas systems can function independently in crRNA biogenesis within a given organism ? an important consideration in engineering co-existing CRISPR-Cas pathways. Twit Text FOAVip The three major types of CRISPR-Cas systems function independently in CRISPR RNA biogenesis in Streptococcus thermophilus ????Mol Microbiol http://www.kidney.de/pget.php?&tw=1&pmid=24811454 #FOAvip Twit Text # Free Paper on # # # # # LINK http://www.kidney.de/pget.php?&tw=1&pmid=24811454 #FOAvip English Wikipedia <ref>{{Cite pmid|24811454}}</ref> The three major types of CRISPR-Cas systems function independently in CRISPR RNA biogenesis in Streptococcus thermophilus #MMPMID24811454 Carte J; Christopher RT; Smith JT; Olson S; Barrangou R; Moineau S; Glover CVC; Graveley BR; Terns RM; Terns MP Mol Microbiol 2014[Jul]; 93 (1): 98-112 PMID24811454show ga CRISPR-Cas systems are small RNA-based immune systems that protect prokaryotes from invaders such as viruses and plasmids. We have investigated the features and biogenesis of the CRISPR (cr)RNAs in Streptococcus thermophilus (Sth) strain DGCC7710, which possesses four different CRISPR-Cas systems including representatives from the three major types of CRISPR-Cas systems. Our results indicate that the crRNAs from each CRISPR locus are specifically processed into divergent crRNA species by Cas proteins (and non-coding RNAs) associated with the respective locus. We find that the Csm Type III-A and Cse Type I-E crRNAs are specifically processed by Cas6 and Cse3 (Cas6e), respectively, and retain an 8-nucleotide CRISPR repeat sequence tag 5? of the invader-targeting sequence. The Cse Type I-E crRNAs also retain a 21-nucleotide 3? repeat tag. The crRNAs from the two Csn Type II-A systems in Sth consist of a 5?-truncated targeting sequence and a 3? tag; however these are distinct in size between the two. Moreover, the Csn1 (Cas9) protein associated with one Csn locus functions specifically in the production of crRNAs from that locus. Our findings indicate that multiple CRISPR-Cas systems can function independently in crRNA biogenesis within a given organism ? an important consideration in engineering co-existing CRISPR-Cas pathways. äThe three major types of CRISPR-Cas systems function independently in (epmc).pdf DeepDyve Pubget Overpricing