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2014 ; 87
(1038
): 20130670
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Three-dimensional visualization of rat brain microvasculature following permanent
focal ischaemia by synchrotron radiation
#MMPMID24702152
Zhang MQ
; Sun DN
; Xie YY
; Peng GY
; Xia J
; Long HY
; Xiao B
Br J Radiol
2014[Jun]; 87
(1038
): 20130670
PMID24702152
show ga
OBJECTIVE: Identifying morphological changes that occur in microvessels under
both normal and ischaemic conditions is crucial for understanding and treating
stroke. However, conventional imaging techniques are not able to detect
microvessels on a micron or sub-micron scale without angiography. In the present
study, synchrotron radiation (SR)-based X-ray in-line phase contrast imaging
(ILPCI) was used to acquire high-resolution and high-contrast images of rat brain
tissues in both normal and ischaemic states. METHODS: ILPCI was performed at the
Shanghai Synchrotron Radiation Facility, Shanghai, China, without the use of
contrast agents. CT slices were reformatted and then converted into
three-dimensional (3D) reconstruction images to analyse subtle details of the
cerebral microvascular network. RESULTS: By using ILPCI, brain vessels up to 11.8
?m in diameter were resolved. The number of cortical and penetrating arteries
detected were found to undergo a remarkable decrease within the infarct area. 3
days after permanent ischaemia, vascular masses were also observed in the
peripheral region of the infarcts. CONCLUSION: SR-based ILPCI-CT can serve as a
powerful tool to accurately visualize brain microvasculature. The morphological
parameters of blood vessels in both CT slices and 3D reconstructions were
determined, and this approach has great potential for providing an effective
diagnosis and evaluation for rehabilitation therapy for stroke. ADVANCES IN
KNOWLEDGE: In the absence of contrast agent, the 3D morphologies of the brain
microvasculature in normal and stroke rats were obtained using SR-based ILPCI. SR
imaging is a sensitive and promising method which can be used to explore primary
brain function.