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10.1016/j.cub.2014.04.032 http://scihub22266oqcxt.onion/10.1016/j.cub.2014.04.032 C4075064!4075064!24909324     free     free     free Deprecated: Implicit conversion from float 227.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 227.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534       Curr+Biol 2014 ; 24 (12): 1341-6 Nephropedia Template TP {{tp|p=24909324|t=2014. Zelda potentiates morphogen activity by increasing chromatin accessibility |pdf=|usr=}}{{24909324}} gab.com Text Zelda potentiates morphogen activity by increasing chromatin accessibility JO: Curr Biol http://www.kidney.de/pget.php?&tw=1&pmid=24909324 #FOAvip Zygotic genome activation (ZGA) is a major genome programming event whereby the cells of the embryo begin to adopt specified fates. Experiments in Drosophila and zebrafish revealed that ZGA depends on transcription factors that provide large-scale control of gene expression by direct and specific binding to gene regulatory sequences [1?5]. Zelda (Zld) plays such a role in the Drosophila embryo, where it was shown to control the action of patterning signals [1, 2], however the mechanisms underlying this effect remain largely unclear. A recent model proposed that Zld binding sites act as quantitative regulators of the spatiotemporal expression of genes activated by Dorsal (Dl), the morphogen that patterns the dorsoventral (DV) axis [6]. We test this model experimentally, using enhancers of brinker (brk) and short gastrulation (sog), both of which are directly activated by Dl, but at different concentration thresholds [7?9]. In agreement with the model, we show that there is a clear positive correlation between the number of Zld binding sites and the spatial domain of enhancer activity. Likewise, the timing of expression could be advanced or delayed. We present evidence that Zld facilitates binding of Dl to regulatory DNA, and that this is associated with increased chromatin accessibility. Importantly, the change in chromatin accessibility is strongly correlated with the change in Zld binding, but not Dl. We propose that the ability of genome activators to facilitate read-out of transcriptional input is key to widespread transcriptional induction during ZGA. Twit Text FOAVip Zelda potentiates morphogen activity by increasing chromatin accessibility ????Curr Biol http://www.kidney.de/pget.php?&tw=1&pmid=24909324 #FOAvip Twit Text # Free Paper on # # # # # LINK http://www.kidney.de/pget.php?&tw=1&pmid=24909324 #FOAvip English Wikipedia <ref>{{Cite pmid|24909324}}</ref> Zelda potentiates morphogen activity by increasing chromatin accessibility #MMPMID24909324 Foo SM; Sun Y; Lim B; Ziukaite R; O?Brien K; Nien CY; Kirov N; Shvartsman SY; Rushlow CA Curr Biol 2014[Jun]; 24 (12): 1341-6 PMID24909324show ga Zygotic genome activation (ZGA) is a major genome programming event whereby the cells of the embryo begin to adopt specified fates. Experiments in Drosophila and zebrafish revealed that ZGA depends on transcription factors that provide large-scale control of gene expression by direct and specific binding to gene regulatory sequences [1?5]. Zelda (Zld) plays such a role in the Drosophila embryo, where it was shown to control the action of patterning signals [1, 2], however the mechanisms underlying this effect remain largely unclear. A recent model proposed that Zld binding sites act as quantitative regulators of the spatiotemporal expression of genes activated by Dorsal (Dl), the morphogen that patterns the dorsoventral (DV) axis [6]. We test this model experimentally, using enhancers of brinker (brk) and short gastrulation (sog), both of which are directly activated by Dl, but at different concentration thresholds [7?9]. In agreement with the model, we show that there is a clear positive correlation between the number of Zld binding sites and the spatial domain of enhancer activity. Likewise, the timing of expression could be advanced or delayed. We present evidence that Zld facilitates binding of Dl to regulatory DNA, and that this is associated with increased chromatin accessibility. Importantly, the change in chromatin accessibility is strongly correlated with the change in Zld binding, but not Dl. We propose that the ability of genome activators to facilitate read-out of transcriptional input is key to widespread transcriptional induction during ZGA. äZelda potentiates morphogen activity by increasing chromatin accessibi(epmc).pdf DeepDyve Pubget Overpricing