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Orione, a web-based framework for NGS analysis in microbiology #MMPMID24618473
Cuccuru G; Orsini M; Pinna A; Sbardellati A; Soranzo N; Travaglione A; Uva P; Zanetti G; Fotia G
Bioinformatics 2014[Jul]; 30 (13): 1928-9 PMID24618473show ga
Summary: End-to-end next-generation sequencing microbiology data analysis requires a diversity of tools covering bacterial resequencing, de novo assembly, scaffolding, bacterial RNA-Seq, gene annotation and metagenomics. However, the construction of computational pipelines that use different software packages is difficult owing to a lack of interoperability, reproducibility and transparency. To overcome these limitations we present Orione, a Galaxy-based framework consisting of publicly available research software and specifically designed pipelines to build complex, reproducible workflows for next-generation sequencing microbiology data analysis. Enabling microbiology researchers to conduct their own custom analysis and data manipulation without software installation or programming, Orione provides new opportunities for data-intensive computational analyses in microbiology and metagenomics.Availability and implementation: Orione is available online at http://orione.crs4.it.Contact:gianmauro.cuccuru@crs4.itSupplementary information:Supplementary data are available at Bioinformatics online.