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http://scihub22266oqcxt.onion/ C4069922!4069922!24966897     free     free     free Deprecated: Implicit conversion from float 211.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 211.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 211.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 211.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 211.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 211.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 211.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 211.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 245.2 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534       Int+J+Clin+Exp+Pathol 2014 ; 7 (5): 1868-79 Nephropedia Template TP {{tp|p=24966897|t=2014. Differentiation induction of mouse cardiac stem cells into sinus node-like cells by co-culturing with sinus node |pdf=|usr=}}{{24966897}} gab.com Text Differentiation induction of mouse cardiac stem cells into sinus node-like cells by co-culturing with sinus node JO: Int J Clin Exp Pathol http://www.kidney.de/pget.php?&tw=1&pmid=24966897 #FOAvip Sinus nodal cells can generate a diastolic or ?pacemaker? depolarization at the end of an action potential driving the membrane potential slowly up to the threshold for firing the next action potential. It has been proved that adult cardiac stem cells (CSCs) can differentiate into sinus nodal cells by demethylating agent. However, there is no report about adult CSCs-derived sinus nodal cells with pacemaker current (the funny current, If). In this study, we isolated the mouse adult CSCs from mouse hearts by the method of tissue explants adherence. The expression of c-kit protein indicated the isolation of CSCs. Then we co-cultured mouse CSCs with mouse sinus node tissue to induce the differentiation of these CSCs into sinus node-like cells, which was proved by identifying the enhanced expression of marker proteins cTnI, cTnT and ?-Actinin with Immunofluorescence staining. At the same time, with whole-cell patch-clamp we detected the If current, which can be blocked by CsCl, in these differentiated cells. In conclusion, by confirming specific If current in the induced node-like cells, our work shows a method inducing differentiation of CSCs into sinus node-like cells, which can provide helpful information for the further research on sick sinus syndrome. Twit Text FOAVip Differentiation induction of mouse cardiac stem cells into sinus node-like cells by co-culturing with sinus node ????Int J Clin Exp Pathol http://www.kidney.de/pget.php?&tw=1&pmid=24966897 #FOAvip Twit Text # Free Paper on # # # # # LINK http://www.kidney.de/pget.php?&tw=1&pmid=24966897 #FOAvip English Wikipedia <ref>{{Cite pmid|24966897}}</ref> Differentiation induction of mouse cardiac stem cells into sinus node-like cells by co-culturing with sinus node #MMPMID24966897 Fang YB; Liu X; Wen J; Tang XJ; Yu FX; Deng MB; Wu CX; Liao B Int J Clin Exp Pathol 2014[]; 7 (5): 1868-79 PMID24966897show ga Sinus nodal cells can generate a diastolic or ?pacemaker? depolarization at the end of an action potential driving the membrane potential slowly up to the threshold for firing the next action potential. It has been proved that adult cardiac stem cells (CSCs) can differentiate into sinus nodal cells by demethylating agent. However, there is no report about adult CSCs-derived sinus nodal cells with pacemaker current (the funny current, If). In this study, we isolated the mouse adult CSCs from mouse hearts by the method of tissue explants adherence. The expression of c-kit protein indicated the isolation of CSCs. Then we co-cultured mouse CSCs with mouse sinus node tissue to induce the differentiation of these CSCs into sinus node-like cells, which was proved by identifying the enhanced expression of marker proteins cTnI, cTnT and ?-Actinin with Immunofluorescence staining. At the same time, with whole-cell patch-clamp we detected the If current, which can be blocked by CsCl, in these differentiated cells. In conclusion, by confirming specific If current in the induced node-like cells, our work shows a method inducing differentiation of CSCs into sinus node-like cells, which can provide helpful information for the further research on sick sinus syndrome. äDifferentiation induction of mouse cardiac stem cells into sinus node-(epmc).pdf DeepDyve Pubget Overpricing