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10.1074/jbc.M114.576827 http://scihub22266oqcxt.onion/10.1074/jbc.M114.576827 C4067212!4067212 !24808183     free     free     free Deprecated: Implicit conversion from float 211.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 211.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 211.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 211.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 211.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 211.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 211.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 211.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 245.2 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 245.2 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 245.2 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Warning: imagejpeg(C:\Inetpub\vhosts\kidney.de\httpdocs\phplern\24808183 .jpg): Failed to open stream: No such file or directory in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 117       J+Biol+Chem 2014 ; 289 (25 ): 17791-801 Nephropedia Template TP {{tp|p=24808183 |t=2014. A chemical biology approach demonstrates G protein ?? subunits are sufficient to mediate directional neutrophil chemotaxis |pdf=|usr=}}{{24808183 }} gab.com Text A chemical biology approach demonstrates G protein subunits are sufficient to mediate directional neutrophil chemotaxis JO: J Biol Chem http://www.kidney.de/pget.php?&tw=1&pmid=24808183 #FOAvip Our laboratory has identified a number of small molecules that bind to G protein ?? subunits (G??) by competing for peptide binding to the G?? "hot spot." M119/Gallein were identified as inhibitors of G?? subunit signaling. Here we examine the activity of another molecule identified in this screen, 12155, which we show that in contrast to M119/Gallein had no effect on G??-mediated phospholipase C or phosphoinositide 3-kinase (PI3K) ? activation in vitro. Also in direct contrast to M119/Gallein, 12155 caused receptor-independent Ca(2+) release, and activated other downstream targets of G?? including extracellular signal regulated kinase (ERK), protein kinase B (Akt) in HL60 cells differentiated to neutrophils. We show that 12155 releases G?? in vitro from G?i1?1?2 heterotrimers by causing its dissociation from G?GDP without inducing nucleotide exchange in the G? subunit. We used this novel probe to examine the hypothesis that G?? release is sufficient to direct chemotaxis of neutrophils in the absence of receptor or G protein ? subunit activation. 12155 directed chemotaxis of HL60 cells and primary neutrophils in a transwell migration assay with responses similar to those seen for the natural chemotactic peptide n-formyl-Met-Leu-Phe. These data indicate that release of free G?? is sufficient to drive directional chemotaxis in a G protein-coupled receptor signaling-independent manner. Twit Text FOAVip A chemical biology approach demonstrates G protein subunits are sufficient to mediate directional neutrophil chemotaxis ????J Biol Chem http://www.kidney.de/pget.php?&tw=1&pmid=24808183 #FOAvip Twit Text # Free Paper on # # # # # LINK http://www.kidney.de/pget.php?&tw=1&pmid=24808183 #FOAvip English Wikipedia <ref>{{Cite pmid|24808183 }}</ref> A chemical biology approach demonstrates G protein ?? subunits are sufficient to mediate directional neutrophil chemotaxis #MMPMID24808183 Surve CR ; Lehmann D ; Smrcka AV J Biol Chem 2014[Jun]; 289 (25 ): 17791-801 PMID24808183 show ga Our laboratory has identified a number of small molecules that bind to G protein ?? subunits (G??) by competing for peptide binding to the G?? "hot spot." M119/Gallein were identified as inhibitors of G?? subunit signaling. Here we examine the activity of another molecule identified in this screen, 12155, which we show that in contrast to M119/Gallein had no effect on G??-mediated phospholipase C or phosphoinositide 3-kinase (PI3K) ? activation in vitro. Also in direct contrast to M119/Gallein, 12155 caused receptor-independent Ca(2+) release, and activated other downstream targets of G?? including extracellular signal regulated kinase (ERK), protein kinase B (Akt) in HL60 cells differentiated to neutrophils. We show that 12155 releases G?? in vitro from G?i1?1?2 heterotrimers by causing its dissociation from G?GDP without inducing nucleotide exchange in the G? subunit. We used this novel probe to examine the hypothesis that G?? release is sufficient to direct chemotaxis of neutrophils in the absence of receptor or G protein ? subunit activation. 12155 directed chemotaxis of HL60 cells and primary neutrophils in a transwell migration assay with responses similar to those seen for the natural chemotactic peptide n-formyl-Met-Leu-Phe. These data indicate that release of free G?? is sufficient to drive directional chemotaxis in a G protein-coupled receptor signaling-independent manner. |Animals [MESH] |Calcium/metabolism [MESH] |Chemotaxis/drug effects/*physiology [MESH] |Cyclohexanes/chemistry/pharmacology [MESH] |Extracellular Signal-Regulated MAP Kinases/genetics/metabolism [MESH] |GTP-Binding Protein alpha Subunits, Gi-Go/genetics/metabolism [MESH] |GTP-Binding Protein beta Subunits/antagonists & inhibitors/genetics/*metabolism [MESH] |GTP-Binding Protein gamma Subunits/antagonists & inhibitors/genetics/*metabolism [MESH] |HL-60 Cells [MESH] |Humans [MESH] |Mice [MESH] |N-Formylmethionine Leucyl-Phenylalanine/pharmacology [MESH] |Neutrophils/cytology/*metabolism [MESH] |Proto-Oncogene Proteins c-akt/genetics/metabolism [MESH] |Signal Transduction/drug effects/*physiology [MESH]A chemical biology approach demonstrates G protein ?? subunits are suf(epmc).pdf DeepDyve Pubget Overpricing