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10.1016/j.ab.2008.04.042 http://scihub22266oqcxt.onion/10.1016/j.ab.2008.04.042 C4065382!4065382!18486587     free     free     free Deprecated: Implicit conversion from float 211.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 211.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 211.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 211.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 211.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 211.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534       Anal+Biochem 2008 ; 379 (1): 40-4 Nephropedia Template TP {{tp|p=18486587|t=2008. Estimating glutathione synthesis with deuterated water: A model for peptide biosynthesis |pdf=|usr=}}{{18486587}} gab.com Text Estimating glutathione synthesis with deuterated water: A model for peptide biosynthesis JO: Anal Biochem http://www.kidney.de/pget.php?&tw=1&pmid=18486587 #FOAvip Glutathione (GSH), an intracellular tripeptide that combats oxidative stress, must be continually replaced due to loss through conjugation and destruction. Previous methods, estimating the synthesis of GSH in vivo, used constant infusions of labeled amino acid precursors. We developed a new method based on incorporation of 2H from orally supplied 2H2O into stable C-H bonds on the tripeptide. The incorporation of 2H2O into GSH was studied in rabbits over a two week period. The method estimated N, the maximum number of C-H bonds in GSH that equilibrate with 2H2O as amino acids. GSH was analyzed by liquid chromatography mass spectrometry after derivatization to yield GSH-N-ethylmaleimide (GSNEM). A model, which simulated the expected abundance at each mass isotopomer for the GSNEM ion at various values for N, was used to find the best fit to the data. The plateau labeling fit best a model with N= 6 of a possible 10 C-H bonds. Thus, the amino acids precursors do not completely equilibrate with 2H2O prior to GSH synthesis. Advantages of this new method include replacing costly amino acid infusions with the oral administration of 2H2O and a statistical basis for estimating N. Twit Text FOAVip Estimating glutathione synthesis with deuterated water: A model for peptide biosynthesis ????Anal Biochem http://www.kidney.de/pget.php?&tw=1&pmid=18486587 #FOAvip Twit Text # Free Paper on # # # # # LINK http://www.kidney.de/pget.php?&tw=1&pmid=18486587 #FOAvip English Wikipedia <ref>{{Cite pmid|18486587}}</ref> Estimating glutathione synthesis with deuterated water: A model for peptide biosynthesis #MMPMID18486587 Cabral CB; Bullock KH; Bischoff DJ; Tompkins RG; Yu YM; Kelleher JK Anal Biochem 2008[Aug]; 379 (1): 40-4 PMID18486587show ga Glutathione (GSH), an intracellular tripeptide that combats oxidative stress, must be continually replaced due to loss through conjugation and destruction. Previous methods, estimating the synthesis of GSH in vivo, used constant infusions of labeled amino acid precursors. We developed a new method based on incorporation of 2H from orally supplied 2H2O into stable C-H bonds on the tripeptide. The incorporation of 2H2O into GSH was studied in rabbits over a two week period. The method estimated N, the maximum number of C-H bonds in GSH that equilibrate with 2H2O as amino acids. GSH was analyzed by liquid chromatography mass spectrometry after derivatization to yield GSH-N-ethylmaleimide (GSNEM). A model, which simulated the expected abundance at each mass isotopomer for the GSNEM ion at various values for N, was used to find the best fit to the data. The plateau labeling fit best a model with N= 6 of a possible 10 C-H bonds. Thus, the amino acids precursors do not completely equilibrate with 2H2O prior to GSH synthesis. Advantages of this new method include replacing costly amino acid infusions with the oral administration of 2H2O and a statistical basis for estimating N. äEstimating glutathione synthesis with deuterated water: A model for pe(epmc).pdf DeepDyve Pubget Overpricing