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2014 ; 15
(ä): 128
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English Wikipedia
BLASTGrabber: a bioinformatic tool for visualization, analysis and sequence
selection of massive BLAST data
#MMPMID24885091
Neumann RS
; Kumar S
; Haverkamp TH
; Shalchian-Tabrizi K
BMC Bioinformatics
2014[May]; 15
(ä): 128
PMID24885091
show ga
BACKGROUND: Advances in sequencing efficiency have vastly increased the sizes of
biological sequence databases, including many thousands of genome-sequenced
species. The BLAST algorithm remains the main search engine for retrieving
sequence information, and must consequently handle data on an unprecedented
scale. This has been possible due to high-performance computers and parallel
processing. However, the raw BLAST output from contemporary searches involving
thousands of queries becomes ill-suited for direct human processing. Few programs
attempt to directly visualize and interpret BLAST output; those that do often
provide a mere basic structuring of BLAST data. RESULTS: Here we present a
bioinformatics application named BLASTGrabber suitable for high-throughput
sequencing analysis. BLASTGrabber, being implemented as a Java application, is
OS-independent and includes a user friendly graphical user interface. Text or
XML-formatted BLAST output files can be directly imported, displayed and
categorized based on BLAST statistics. Query names and FASTA headers can be
analysed by text-mining. In addition to visualizing sequence alignments, BLAST
data can be ordered as an interactive taxonomy tree. All modes of analysis
support selection, export and storage of data. A Java interface-based plugin
structure facilitates the addition of customized third party functionality.
CONCLUSION: The BLASTGrabber application introduces new ways of visualizing and
analysing massive BLAST output data by integrating taxonomy identification, text
mining capabilities and generic multi-dimensional rendering of BLAST hits. The
program aims at a non-expert audience in terms of computer skills; the
combination of new functionalities makes the program flexible and useful for a
broad range of operations.