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10.1021/am501309d

http://scihub22266oqcxt.onion/10.1021/am501309d
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C4059262!4059262!24749832
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suck abstract from ncbi


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pmid24749832      ACS+Appl+Mater+Interfaces 2014 ; 6 (11): 8401-6
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  • ?Click? Immobilization of a VEGF-Mimetic Peptide on Decellularized Endothelial Extracellular Matrix to Enhance Angiogenesis #MMPMID24749832
  • Wang L; Zhao M; Li S; Erasquin UJ; Wang H; Ren L; Chen C; Wang Y; Cai C
  • ACS Appl Mater Interfaces 2014[Jun]; 6 (11): 8401-6 PMID24749832show ga
  • We show that coating of decellularized extracellular matrix (DC-ECM) on substrate surfaces is an efficient way to generate a platform mimicking the native ECM environment. Moreover, the DC-ECM can be modified with a peptide (QK) mimicking vascular endothelial growth factor without apparently compromising its integrity. The modification was achieved through metabolic incorporation of a ?clickable? handle to DC-ECM followed by rapid attachment of the QK peptide with an azido tag using copper-catalyzed click reaction. The attachment of the QK peptide on to DC-ECM in this way further enhanced the angiogenic responses (formation of branched tubular networks) of endothelial cells.
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