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2014 ; 2014
(ä): 647238
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Clinical comparisons of two free light chain assays to immunofixation
electrophoresis for detecting monoclonal gammopathy
#MMPMID24971342
Kim HS
; Kim HS
; Shin KS
; Song W
; Kim HJ
; Kim HS
; Park MJ
Biomed Res Int
2014[]; 2014
(ä): 647238
PMID24971342
show ga
Free light chains (FLCs) are useful biomarkers for the diagnosis and monitoring
of various plasma cell dyscrasias. One hundred fifty-seven samples from 120
patients for screening or monitoring of monoclonal gammopathy (MG) were included.
The new N Latex FLC assays (Siemens Healthcare Diagnostics GmbH, Germany) were
compared with the Freelite FLC assays (The Binding Site Ltd., UK) and the results
were analyzed with those of immunofixation electrophoresis (IFE). The Freelite
FLC assay showed significantly wider assay ranges than the N Latex FLC assay. The
correlation coefficients of the two FLC kappa (?) assays, lambda (?) assays, and
the ?/? ratio were 0.9792, 0.8264, and 0.9064, respectively. The concordance rate
was 84.7% for the FLC ? assays, 79.6% for FLC ?, and 89.2% for the ?/? ratio. The
clinical sensitivity and specificity of the ?/? ratios were 72.2% and 93.6% for
the Freelite assay and 64.6% and 100% for the N Latex FLC assay. Two FLC assays
showed good correlations and concordance. However, the clinical sensitivity of
the ?/? ratio was higher in the Freelite FLC assays; clinical specificity was
higher in the N Latex FLC assay. Both FLC assays seem to have limited clinical
utility in detecting MG in certain clinical settings.