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http://scihub22266oqcxt.onion/ C4057834!4057834 !24955155     free     free     free Warning: file_get_contents(https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pubmed&id=24955155 &cmd=llinks): Failed to open stream: HTTP request failed! 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Transforming growth factor-?1 regulates epithelial-mesenchymal transition in association with cancer stem-like cells in a breast cancer cell line |pdf=|usr=}}{{24955155 }} gab.com Text Transforming growth factor- 1 regulates epithelial-mesenchymal transition in association with cancer stem-like cells in a breast cancer cell line JO: Int J Clin Exp Med http://www.kidney.de/pget.php?&tw=1&pmid=24955155 #FOAvip Epithelial-mesenchymal transition (EMT) is associated with altered connection and junctions between cells and changes in abilities of invasion and migration. In this study, we investigated whether SK-BR-3 breast cancer cells induced to undergo EMT exhibit changes in morphological and invasion abilities after Transforming growth factor ?1 (TGF-?1) treatment. Serum-deprived SK-BR-3 cells were treated with TGF-?1 (0, 10 ng/mL) for 24 h. The cells morphological changes were observed and imaged using inverted phase contrast microscope. Scratch experiment and invasion experiment were employed to detect changes of invasion ability, cell-flow experiment was used to assess cell cycle, immunohistochemistry technique was used to detect epithelial and mesenchymal markers after the crawling cells were fixed. Our research reveal that SK-BR-3 cells become larger and more messy, the elongated cells extend pseudopodia, the link of the cells became more loosely and cell gap widened after TGF-?1 treatment. SK-BR-3 cells showed faster growing and improved invasion abilities after TGF-?1 treatment, and reduced G1 phase cells proportion in the total number of cells after the conversion, in contrast the S phase cells accounted for the proportion of the total number of cells increased. These findings indicate that TGF-?1-induced EMT in breast cancer cells may be associated with major alterations in morphological and invasion abilities. Twit Text FOAVip Transforming growth factor- 1 regulates epithelial-mesenchymal transition in association with cancer stem-like cells in a breast cancer cell line ????Int J Clin Exp Med http://www.kidney.de/pget.php?&tw=1&pmid=24955155 #FOAvip Twit Text # Free Paper on # # # # # LINK http://www.kidney.de/pget.php?&tw=1&pmid=24955155 #FOAvip English Wikipedia <ref>{{Cite pmid|24955155 }}</ref> Transforming growth factor-?1 regulates epithelial-mesenchymal transition in association with cancer stem-like cells in a breast cancer cell line #MMPMID24955155 Jia Y ; Wu D ; Yun F ; Shi L ; Luo N ; Liu Z ; Shi Y ; Sun Q ; Jiang L ; Wang S ; Du M Int J Clin Exp Med 2014[]; 7 (4 ): 865-72 PMID24955155 show ga Epithelial-mesenchymal transition (EMT) is associated with altered connection and junctions between cells and changes in abilities of invasion and migration. In this study, we investigated whether SK-BR-3 breast cancer cells induced to undergo EMT exhibit changes in morphological and invasion abilities after Transforming growth factor ?1 (TGF-?1) treatment. Serum-deprived SK-BR-3 cells were treated with TGF-?1 (0, 10 ng/mL) for 24 h. The cells morphological changes were observed and imaged using inverted phase contrast microscope. Scratch experiment and invasion experiment were employed to detect changes of invasion ability, cell-flow experiment was used to assess cell cycle, immunohistochemistry technique was used to detect epithelial and mesenchymal markers after the crawling cells were fixed. Our research reveal that SK-BR-3 cells become larger and more messy, the elongated cells extend pseudopodia, the link of the cells became more loosely and cell gap widened after TGF-?1 treatment. SK-BR-3 cells showed faster growing and improved invasion abilities after TGF-?1 treatment, and reduced G1 phase cells proportion in the total number of cells after the conversion, in contrast the S phase cells accounted for the proportion of the total number of cells increased. These findings indicate that TGF-?1-induced EMT in breast cancer cells may be associated with major alterations in morphological and invasion abilities. äTransforming growth factor-?1 regulates epithelial-mesenchymal transit(epmc).pdf DeepDyve Pubget Overpricing