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10.1186/gb-2013-14-11-r124 http://scihub22266oqcxt.onion/10.1186/gb-2013-14-11-r124 C4053851!4053851 !24200198     free     free     free Deprecated: Implicit conversion from float 211.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 211.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 211.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 211.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 211.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 245.2 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 245.2 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Warning: imagejpeg(C:\Inetpub\vhosts\kidney.de\httpdocs\phplern\24200198 .jpg): Failed to open stream: No such file or directory in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 117       Genome+Biol 2013 ; 14 (11 ): R124 Nephropedia Template TP {{tp|p=24200198 |t=2013. AHT-ChIP-seq: a completely automated robotic protocol for high-throughput chromatin immunoprecipitation |pdf=|usr=}}{{24200198 }} gab.com Text AHT-ChIP-seq: a completely automated robotic protocol for high-throughput chromatin immunoprecipitation JO: Genome Biol http://www.kidney.de/pget.php?&tw=1&pmid=24200198 #FOAvip ChIP-seq is an established manually-performed method for identifying DNA-protein interactions genome-wide. Here, we describe a protocol for automated high-throughput (AHT) ChIP-seq. To demonstrate the quality of data obtained using AHT-ChIP-seq, we applied it to five proteins in mouse livers using a single 96-well plate, demonstrating an extremely high degree of qualitative and quantitative reproducibility among biological and technical replicates. We estimated the optimum and minimum recommended cell numbers required to perform AHT-ChIP-seq by running an additional plate using HepG2 and MCF7 cells. With this protocol, commercially available robotics can perform four hundred experiments in five days. Twit Text FOAVip AHT-ChIP-seq: a completely automated robotic protocol for high-throughput chromatin immunoprecipitation ????Genome Biol http://www.kidney.de/pget.php?&tw=1&pmid=24200198 #FOAvip Twit Text # Free Paper on # # # # # LINK http://www.kidney.de/pget.php?&tw=1&pmid=24200198 #FOAvip English Wikipedia <ref>{{Cite pmid|24200198 }}</ref> AHT-ChIP-seq: a completely automated robotic protocol for high-throughput chromatin immunoprecipitation #MMPMID24200198 Aldridge S ; Watt S ; Quail MA ; Rayner T ; Lukk M ; Bimson MF ; Gaffney D ; Odom DT Genome Biol 2013[Nov]; 14 (11 ): R124 PMID24200198 show ga ChIP-seq is an established manually-performed method for identifying DNA-protein interactions genome-wide. Here, we describe a protocol for automated high-throughput (AHT) ChIP-seq. To demonstrate the quality of data obtained using AHT-ChIP-seq, we applied it to five proteins in mouse livers using a single 96-well plate, demonstrating an extremely high degree of qualitative and quantitative reproducibility among biological and technical replicates. We estimated the optimum and minimum recommended cell numbers required to perform AHT-ChIP-seq by running an additional plate using HepG2 and MCF7 cells. With this protocol, commercially available robotics can perform four hundred experiments in five days. |Animals [MESH] |Chromatin Immunoprecipitation/instrumentation/*methods [MESH] |Hep G2 Cells [MESH] |High-Throughput Nucleotide Sequencing/instrumentation/*methods [MESH] |Humans [MESH] |Liver/metabolism [MESH] |MCF-7 Cells [MESH] |Male [MESH] |Mice [MESH] |Mice, Inbred C57BL [MESH] |Reproducibility of Results [MESH] |Robotics/instrumentation/*methods [MESH]AHT-ChIP-seq: a completely automated robotic protocol for high-through(epmc).pdf DeepDyve Pubget Overpricing