Warning: file_get_contents(https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pubmed&id=24949451
&cmd=llinks): Failed to open stream: HTTP request failed! HTTP/1.1 429 Too Many Requests
in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 215
Punicalagin and ellagic acid demonstrate antimutagenic activity and inhibition of
benzo a pyrene induced DNA adducts
#MMPMID24949451
Zahin M
; Ahmad I
; Gupta RC
; Aqil F
Biomed Res Int
2014[]; 2014
(?): 467465
PMID24949451
show ga
Punicalagin (PC) is an ellagitannin found in the fruit peel of Punica granatum.
We have demonstrated antioxidant and antigenotoxic properties of Punica granatum
and showed that PC and ellagic acid (EA) are its major constituents. In this
study, we demonstrate the antimutagenic potential, inhibition of BP-induced DNA
damage, and antiproliferative activity of PC and EA. Incubation of BP with rat
liver microsomes, appropriate cofactors, and DNA in the presence of vehicle or PC
and EA showed significant inhibition of the resultant DNA adducts, with
essentially complete inhibition (97%) at 40 ? M by PC and 77% inhibition by EA.
Antimutagenicity was tested by Ames test. PC and EA dose-dependently and markedly
antagonized the effect of tested mutagens, sodium azide, methyl methanesulfonate,
benzo[a]pyrene, and 2-aminoflourine, with maximum inhibition of mutagenicity up
to 90 percent. Almost all the doses tested (50-500 ? M) exhibited significant
antimutagenicity. A profound antiproliferative effect on human lung cancer cells
was also shown with PC and EA. Together, our data show that PC and EA are
pomegranate bioactives responsible for inhibition of BP-induced DNA adducts and
strong antimutagenic, antiproliferative activities. However, these compounds are
to be evaluated in suitable animal model to assess their therapeutic efficacy
against cancer.
free
free
free
