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2014 ; 5
(6
): 1731-43
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In vivo imaging of nanoparticle delivery and tumor microvasculature with
multimodal optical coherence tomography
#MMPMID24940536
Tucker-Schwartz JM
; Beavers KR
; Sit WW
; Shah AT
; Duvall CL
; Skala MC
Biomed Opt Express
2014[Jun]; 5
(6
): 1731-43
PMID24940536
show ga
Current imaging techniques capable of tracking nanoparticles in vivo supply
either a large field of view or cellular resolution, but not both. Here, we
demonstrate a multimodality imaging platform of optical coherence tomography
(OCT) techniques for high resolution, wide field of view in vivo imaging of
nanoparticles. This platform includes the first in vivo images of nanoparticle
pharmacokinetics acquired with photothermal OCT (PTOCT), along with overlaying
images of microvascular and tissue morphology. Gold nanorods (51.8 ± 8.1 nm by
15.2 ± 3.3 nm) were intravenously injected into mice, and their accumulation into
mammary tumors was non-invasively imaged in vivo in three dimensions over 24
hours using PTOCT. Spatial frequency analysis of PTOCT images indicated that gold
nanorods reached peak distribution throughout the tumors by 16 hours, and
remained well-dispersed up to 24 hours post-injection. In contrast, the overall
accumulation of gold nanorods within the tumors peaked around 16 hours
post-injection. The accumulation of gold nanorods within the tumors was validated
post-mortem with multiphoton microscopy. This shows the utility of PTOCT as part
of a powerful multimodality imaging platform for the development of nanomedicines
and drug delivery technologies.