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10.1016/j.bpj.2014.04.011 http://scihub22266oqcxt.onion/10.1016/j.bpj.2014.04.011 C4052276!4052276!24896112     free     free     free Deprecated: Implicit conversion from float 219.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 219.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 219.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 219.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 219.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 219.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534       Biophys+J 2014 ; 106 (11): 2330-9 Nephropedia Template TP {{tp|p=24896112|t=2014. Ultimate Use of Two-Photon Fluorescence Microscopy to Map Orientational Behavior of Fluorophores |pdf=|usr=}}{{24896112}} gab.com Text Ultimate Use of Two-Photon Fluorescence Microscopy to Map Orientational Behavior of Fluorophores JO: Biophys J http://www.kidney.de/pget.php?&tw=1&pmid=24896112 #FOAvip The orientational distribution of fluorophores is an important reporter of the structure and function of their molecular environment. Although this distribution affects the fluorescence signal under polarized-light excitation, its retrieval is limited to a small number of parameters. Because of this limitation, the need for a geometrical model (cone, Gaussian, etc.) to effect such retrieval is often invoked. In this work, using a symmetry decomposition of the distribution function of the fluorescent molecules, we show that polarized two-photon fluorescence based on tunable linear dichroism allows for the retrieval of this distribution with reasonable fidelity and without invoking either an a priori knowledge of the system to be investigated or a geometrical model. We establish the optimal level of detail to which any distribution can be retrieved using this technique. As applied to artificial lipid vesicles and cell membranes, the ability of this method to identify and quantify specific structural properties that complement the more traditional molecular-order information is demonstrated. In particular, we analyze situations that give access to the sharpness of the angular constraint, and to the evidence of an isotropic population of fluorophores within the focal volume encompassing the membrane. Moreover, this technique has the potential to address complex situations such as the distribution of a tethered membrane protein label in an ordered environment. Twit Text FOAVip Ultimate Use of Two-Photon Fluorescence Microscopy to Map Orientational Behavior of Fluorophores ????Biophys J http://www.kidney.de/pget.php?&tw=1&pmid=24896112 #FOAvip Twit Text # Free Paper on # # # # # LINK http://www.kidney.de/pget.php?&tw=1&pmid=24896112 #FOAvip English Wikipedia <ref>{{Cite pmid|24896112}}</ref> Ultimate Use of Two-Photon Fluorescence Microscopy to Map Orientational Behavior of Fluorophores #MMPMID24896112 Ferrand P; Gasecka P; Kress A; Wang X; Bioud FZ; Duboisset J; Brasselet S Biophys J 2014[Jun]; 106 (11): 2330-9 PMID24896112show ga The orientational distribution of fluorophores is an important reporter of the structure and function of their molecular environment. Although this distribution affects the fluorescence signal under polarized-light excitation, its retrieval is limited to a small number of parameters. Because of this limitation, the need for a geometrical model (cone, Gaussian, etc.) to effect such retrieval is often invoked. In this work, using a symmetry decomposition of the distribution function of the fluorescent molecules, we show that polarized two-photon fluorescence based on tunable linear dichroism allows for the retrieval of this distribution with reasonable fidelity and without invoking either an a priori knowledge of the system to be investigated or a geometrical model. We establish the optimal level of detail to which any distribution can be retrieved using this technique. As applied to artificial lipid vesicles and cell membranes, the ability of this method to identify and quantify specific structural properties that complement the more traditional molecular-order information is demonstrated. In particular, we analyze situations that give access to the sharpness of the angular constraint, and to the evidence of an isotropic population of fluorophores within the focal volume encompassing the membrane. Moreover, this technique has the potential to address complex situations such as the distribution of a tethered membrane protein label in an ordered environment. äUltimate Use of Two-Photon Fluorescence Microscopy to Map Orientationa(epmc).pdf DeepDyve Pubget Overpricing