Use my Search Websuite to scan PubMed, PMCentral, Journal Hosts and Journal Archives, FullText. Kick-your-searchterm to multiple Engines kick-your-query now !>

A dictionary by aggregated review articles of nephrology, medicine and the life sciences Your one-stop-run pathway from word to the immediate pdf of peer-reviewed on-topic knowledge.

10.1002/anie.201400001 http://scihub22266oqcxt.onion/10.1002/anie.201400001 C4051619!4051619!24615769     free     free     free Deprecated: Implicit conversion from float 213.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 213.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 213.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 213.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 213.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 213.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 213.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534       Angew+Chem+Int+Ed+Engl 2014 ; 53 (15): 3932-6 Nephropedia Template TP {{tp|p=24615769|t=2014. Genetically Encoding Photoswitchable Click Amino Acids in Escherichia coli and Mammalian Cells** |pdf=|usr=}}{{24615769}} gab.com Text Genetically Encoding Photoswitchable Click Amino Acids in Escherichia coli and Mammalian Cells** JO: Angew Chem Int Ed Engl http://www.kidney.de/pget.php?&tw=1&pmid=24615769 #FOAvip The ability to reversibly control protein structure and function with light would offer high spatiotemporal resolution to investigate biological processes. To enable photoresponsiveness to general proteins, we genetically incorporated into proteins a set of photoswitchable click amino acids (PSCaas), which contain both a reversible photoswitch and an additional click functional group for further modifications. Orthogonal tRNA-synthetases were evolved to genetically encode PSCaas bearing azobenzene and alkene, keto or benzylchloride in E. coli and in mammalian cells. The benzylchloride PSCaa, after incorporated into calmodulin, spontaneously generated a covalent protein bridge by reacting with a nearby cysteine via proximity-enabled bioreactivity. The resultant azobenzene bridge isomerized in response to light changing calmodulin conformation. These genetically encodable PSCaas will prove valuable to engineer photoswitchable bridges into proteins for reversible optogenetic regulation. Twit Text FOAVip Genetically Encoding Photoswitchable Click Amino Acids in Escherichia coli and Mammalian Cells** ????Angew Chem Int Ed Engl http://www.kidney.de/pget.php?&tw=1&pmid=24615769 #FOAvip Twit Text # Free Paper on # # # # # LINK http://www.kidney.de/pget.php?&tw=1&pmid=24615769 #FOAvip English Wikipedia <ref>{{Cite pmid|24615769}}</ref> Genetically Encoding Photoswitchable Click Amino Acids in Escherichia coli and Mammalian Cells** #MMPMID24615769 Hoppmann C; Lacey VK; Louie GV; Wei J; Noel JP; Wang L Angew Chem Int Ed Engl 2014[Apr]; 53 (15): 3932-6 PMID24615769show ga The ability to reversibly control protein structure and function with light would offer high spatiotemporal resolution to investigate biological processes. To enable photoresponsiveness to general proteins, we genetically incorporated into proteins a set of photoswitchable click amino acids (PSCaas), which contain both a reversible photoswitch and an additional click functional group for further modifications. Orthogonal tRNA-synthetases were evolved to genetically encode PSCaas bearing azobenzene and alkene, keto or benzylchloride in E. coli and in mammalian cells. The benzylchloride PSCaa, after incorporated into calmodulin, spontaneously generated a covalent protein bridge by reacting with a nearby cysteine via proximity-enabled bioreactivity. The resultant azobenzene bridge isomerized in response to light changing calmodulin conformation. These genetically encodable PSCaas will prove valuable to engineer photoswitchable bridges into proteins for reversible optogenetic regulation. äGenetically Encoding Photoswitchable Click Amino Acids in Escherichia (epmc).pdf DeepDyve Pubget Overpricing