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10.1007/s10815-014-0209-0 http://scihub22266oqcxt.onion/10.1007/s10815-014-0209-0 C4048390!4048390 !24760720     free     free     free Warning: file_get_contents(https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pubmed&id=24760720 &cmd=llinks): Failed to open stream: HTTP request failed! 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Study of the effect of varicocelectomy on sperm proteins expression in patients with varicocele and poor sperm quality by using two-dimensional gel electrophoresis |pdf=|usr=}}{{24760720 }} gab.com Text Study of the effect of varicocelectomy on sperm proteins expression in patients with varicocele and poor sperm quality by using two-dimensional gel electrophoresis JO: J Assist Reprod Genet http://www.kidney.de/pget.php?&tw=1&pmid=24760720 #FOAvip PURPOSE: To compare proteomic profiles of spermatozoa from patients with varicocele and poor sperm quality before and after varicocelectomy. METHODS: This work was designed as a prospective and observational study. The study was based on 20 men with varicocele grade 3 and poor sperm quality undergoing varicocelectomy at the Fertility Unit of Royan institute in 2009. Two semen samples were collected, one before varicocelectomy and the other after surgery. Protein separation was done by two-dimensional protein electrophoresis, and analyzed by gel densitometry and mass spectrometry. Differential sperm protein expression levels were measured by gel densitometry. RESULTS: Comparison of the sperm parameters showed that sperm motility and concentration were increased after varicocelectomy. At the level of protein, a total of 3 protein spots were identified whose expression was significantly lower in sperm samples before varicocelectomy compared with after surgery including heat shock protein A5 (HSPA5), superoxide dismutase 1 (SOD1) and ?-subunit of the catalytic core of mitochondrial adenosine triphosphate synthase (ATP5D). CONCLUSIONS: High grade varicocoele affects sperm protein expression presumably because of increasing testicular temperature. These proteins play essential roles in sperm production, DNA integrity protection, and sperm motility. This novel study demonstrates that varicocelectomy can improve both sperm quality and proteins expression. Twit Text FOAVip Study of the effect of varicocelectomy on sperm proteins expression in patients with varicocele and poor sperm quality by using two-dimensional gel electrophoresis ????J Assist Reprod Genet http://www.kidney.de/pget.php?&tw=1&pmid=24760720 #FOAvip Twit Text # Free Paper on # # # # # LINK http://www.kidney.de/pget.php?&tw=1&pmid=24760720 #FOAvip English Wikipedia <ref>{{Cite pmid|24760720 }}</ref> Study of the effect of varicocelectomy on sperm proteins expression in patients with varicocele and poor sperm quality by using two-dimensional gel electrophoresis #MMPMID24760720 Hosseinifar H ; Sabbaghian M ; Nasrabadi D ; Modarresi T ; Dizaj AV ; Gourabi H ; Gilani MA J Assist Reprod Genet 2014[Jun]; 31 (6 ): 725-9 PMID24760720 show ga PURPOSE: To compare proteomic profiles of spermatozoa from patients with varicocele and poor sperm quality before and after varicocelectomy. METHODS: This work was designed as a prospective and observational study. The study was based on 20 men with varicocele grade 3 and poor sperm quality undergoing varicocelectomy at the Fertility Unit of Royan institute in 2009. Two semen samples were collected, one before varicocelectomy and the other after surgery. Protein separation was done by two-dimensional protein electrophoresis, and analyzed by gel densitometry and mass spectrometry. Differential sperm protein expression levels were measured by gel densitometry. RESULTS: Comparison of the sperm parameters showed that sperm motility and concentration were increased after varicocelectomy. At the level of protein, a total of 3 protein spots were identified whose expression was significantly lower in sperm samples before varicocelectomy compared with after surgery including heat shock protein A5 (HSPA5), superoxide dismutase 1 (SOD1) and ?-subunit of the catalytic core of mitochondrial adenosine triphosphate synthase (ATP5D). CONCLUSIONS: High grade varicocoele affects sperm protein expression presumably because of increasing testicular temperature. These proteins play essential roles in sperm production, DNA integrity protection, and sperm motility. This novel study demonstrates that varicocelectomy can improve both sperm quality and proteins expression. |*Proteomics [MESH] |Electrophoresis, Gel, Two-Dimensional [MESH] |Endoplasmic Reticulum Chaperone BiP [MESH] |Gene Expression Regulation [MESH] |Humans [MESH] |Infertility, Male/*genetics/pathology [MESH] |Male [MESH] |Protein Biosynthesis/*genetics [MESH] |Semen/metabolism [MESH] |Spermatozoa/*metabolism [MESH]Study of the effect of varicocelectomy on sperm proteins expression in(epmc).pdf DeepDyve Pubget Overpricing