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10.1074/jbc.M113.542407 http://scihub22266oqcxt.onion/10.1074/jbc.M113.542407 C4047393!4047393 !24790080     free     free     free Deprecated: Implicit conversion from float 227.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 227.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 227.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 227.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 227.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 227.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 227.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 227.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 227.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 261.2 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 261.2 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 261.2 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Warning: imagejpeg(C:\Inetpub\vhosts\kidney.de\httpdocs\phplern\24790080 .jpg): Failed to open stream: No such file or directory in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 117       J+Biol+Chem 2014 ; 289 (23 ): 16239-51 Nephropedia Template TP {{tp|p=24790080 |t=2014. Regulation of TGF-?1-driven differentiation of human lung fibroblasts: emerging roles of cathepsin B and cystatin C |pdf=|usr=}}{{24790080 }} gab.com Text Regulation of TGF- 1-driven differentiation of human lung fibroblasts: emerging roles of cathepsin B and cystatin C JO: J Biol Chem http://www.kidney.de/pget.php?&tw=1&pmid=24790080 #FOAvip Lung matrix homeostasis partly depends on the fine regulation of proteolytic activities. We examined the expression of human cysteine cathepsins (Cats) and their relative contribution to TGF-?1-induced fibroblast differentiation into myofibroblasts. Assays were conducted using both primary fibroblasts obtained from patients with idiopathic pulmonary fibrosis and human lung CCD-19Lu fibroblasts. Pharmacological inhibition and genetic silencing of Cat B diminished ?-smooth muscle actin expression, delayed fibroblast differentiation, and led to an accumulation of intracellular 50-kDa TGF-?1. Moreover, the addition of Cat B generated a 25-kDa mature form of TGF-?1 in Cat B siRNA-pretreated lysates. Inhibition of Cat B decreased Smad 2/3 phosphorylation but had no effect on p38 MAPK and JNK phosphorylation, indicating that Cat B mostly disturbs TGF-?1-driven canonical Smad signaling pathway. Although mRNA expression of cystatin C was stable, its secretion, which was inhibited by brefeldin A, increased during TGF-?1-induced differentiation of idiopathic pulmonary fibrosis and CCD-19Lu fibroblasts. In addition, cystatin C participated in the control of extracellular Cats, because its gene silencing restored their proteolytic activities. These data support the notion that Cat B participates in lung myofibrogenesis as suggested for stellate cells during liver fibrosis. Moreover, we propose that TGF-?1 promotes fibrosis by driving the effective cystatin C-dependent inhibition of extracellular matrix-degrading Cats. Twit Text FOAVip Regulation of TGF- 1-driven differentiation of human lung fibroblasts: emerging roles of cathepsin B and cystatin C ????J Biol Chem http://www.kidney.de/pget.php?&tw=1&pmid=24790080 #FOAvip Twit Text # Free Paper on # # # # # LINK http://www.kidney.de/pget.php?&tw=1&pmid=24790080 #FOAvip English Wikipedia <ref>{{Cite pmid|24790080 }}</ref> Regulation of TGF-?1-driven differentiation of human lung fibroblasts: emerging roles of cathepsin B and cystatin C #MMPMID24790080 Kasabova M ; Joulin-Giet A ; Lecaille F ; Gilmore BF ; Marchand-Adam S ; Saidi A ; Lalmanach G J Biol Chem 2014[Jun]; 289 (23 ): 16239-51 PMID24790080 show ga Lung matrix homeostasis partly depends on the fine regulation of proteolytic activities. We examined the expression of human cysteine cathepsins (Cats) and their relative contribution to TGF-?1-induced fibroblast differentiation into myofibroblasts. Assays were conducted using both primary fibroblasts obtained from patients with idiopathic pulmonary fibrosis and human lung CCD-19Lu fibroblasts. Pharmacological inhibition and genetic silencing of Cat B diminished ?-smooth muscle actin expression, delayed fibroblast differentiation, and led to an accumulation of intracellular 50-kDa TGF-?1. Moreover, the addition of Cat B generated a 25-kDa mature form of TGF-?1 in Cat B siRNA-pretreated lysates. Inhibition of Cat B decreased Smad 2/3 phosphorylation but had no effect on p38 MAPK and JNK phosphorylation, indicating that Cat B mostly disturbs TGF-?1-driven canonical Smad signaling pathway. Although mRNA expression of cystatin C was stable, its secretion, which was inhibited by brefeldin A, increased during TGF-?1-induced differentiation of idiopathic pulmonary fibrosis and CCD-19Lu fibroblasts. In addition, cystatin C participated in the control of extracellular Cats, because its gene silencing restored their proteolytic activities. These data support the notion that Cat B participates in lung myofibrogenesis as suggested for stellate cells during liver fibrosis. Moreover, we propose that TGF-?1 promotes fibrosis by driving the effective cystatin C-dependent inhibition of extracellular matrix-degrading Cats. |Blotting, Western [MESH] |Cathepsin B/genetics/*physiology [MESH] |Cell Differentiation/*physiology [MESH] |Cells, Cultured [MESH] |Cystatin C/*physiology [MESH] |Fibroblasts/cytology [MESH] |Gene Silencing [MESH] |Humans [MESH] |Lung/*cytology [MESH] |Phosphorylation [MESH] |RNA Interference [MESH] |RNA, Small Interfering [MESH] |Real-Time Polymerase Chain Reaction [MESH] |Signal Transduction [MESH] |Smad Proteins/metabolism [MESH]Regulation of TGF-?1-driven differentiation of human lung fibroblasts:(epmc).pdf DeepDyve Pubget Overpricing