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10.1186/1471-2164-14-855 http://scihub22266oqcxt.onion/10.1186/1471-2164-14-855 C4046740!4046740!24308330     free     free     free Deprecated: Implicit conversion from float 229.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 229.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 229.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 229.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534       BMC+Genomics 2013 ; 14 (1): ä Nephropedia Template TP {{tp|p=24308330|t=2013. A platform independent RNA-Seq protocol for the detection of transcriptome complexity |pdf=|usr=}}{{24308330}} gab.com Text A platform independent RNA-Seq protocol for the detection of transcriptome complexity JO: BMC Genomics http://www.kidney.de/pget.php?&tw=1&pmid=24308330 #FOAvip Background: Recent studies have demonstrated an unexpected complexity of transcription in eukaryotes. The majority of the genome is transcribed and only a little fraction of these transcripts is annotated as protein coding genes and their splice variants. Indeed, most transcripts are the result of antisense, overlapping and non-coding RNA expression. In this frame, one of the key aims of high throughput transcriptome sequencing is the detection of all RNA species present in the cell and the first crucial step for RNA-seq users is represented by the choice of the strategy for cDNA library construction. The protocols developed so far provide the utilization of the entire library for a single sequencing run with a specific platform. Results: We set up a unique protocol to generate and amplify a strand-specific cDNA library representative of all RNA species that may be implemented with all major platforms currently available on the market (Roche 454, Illumina, ABI/SOLiD). Our method is reproducible, fast, easy-to-perform and even allows to start from low input total RNA. Furthermore, we provide a suitable bioinformatics tool for the analysis of the sequences produced following this protocol. Conclusion: We tested the efficiency of our strategy, showing that our method is platform-independent, thus allowing the simultaneous analysis of the same sample with different NGS technologies, and providing an accurate quantitative and qualitative portrait of complex whole transcriptomes. Electronic supplementary material: The online version of this article (doi:10.1186/1471-2164-14-855) contains supplementary material, which is available to authorized users. Twit Text FOAVip A platform independent RNA-Seq protocol for the detection of transcriptome complexity ????BMC Genomics http://www.kidney.de/pget.php?&tw=1&pmid=24308330 #FOAvip Twit Text # Free Paper on # # # # # LINK http://www.kidney.de/pget.php?&tw=1&pmid=24308330 #FOAvip English Wikipedia <ref>{{Cite pmid|24308330}}</ref> A platform independent RNA-Seq protocol for the detection of transcriptome complexity #MMPMID24308330 Calabrese C; Mangiulli M; Manzari C; Paluscio AM; Caratozzolo MF; Marzano F; Kurelac I; D?Erchia AM; D?Elia D; Licciulli F; Liuni S; Picardi E; Attimonelli M; Gasparre G; Porcelli AM; Pesole G; Sbisà E; Tullo A BMC Genomics 2013[]; 14 (1): ä PMID24308330show ga Background: Recent studies have demonstrated an unexpected complexity of transcription in eukaryotes. The majority of the genome is transcribed and only a little fraction of these transcripts is annotated as protein coding genes and their splice variants. Indeed, most transcripts are the result of antisense, overlapping and non-coding RNA expression. In this frame, one of the key aims of high throughput transcriptome sequencing is the detection of all RNA species present in the cell and the first crucial step for RNA-seq users is represented by the choice of the strategy for cDNA library construction. The protocols developed so far provide the utilization of the entire library for a single sequencing run with a specific platform. Results: We set up a unique protocol to generate and amplify a strand-specific cDNA library representative of all RNA species that may be implemented with all major platforms currently available on the market (Roche 454, Illumina, ABI/SOLiD). Our method is reproducible, fast, easy-to-perform and even allows to start from low input total RNA. Furthermore, we provide a suitable bioinformatics tool for the analysis of the sequences produced following this protocol. Conclusion: We tested the efficiency of our strategy, showing that our method is platform-independent, thus allowing the simultaneous analysis of the same sample with different NGS technologies, and providing an accurate quantitative and qualitative portrait of complex whole transcriptomes. Electronic supplementary material: The online version of this article (doi:10.1186/1471-2164-14-855) contains supplementary material, which is available to authorized users. äA platform independent RNA-Seq protocol for the detection of transcrip(epmc).pdf DeepDyve Pubget Overpricing