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10.1089/scd.2013.0497 http://scihub22266oqcxt.onion/10.1089/scd.2013.0497 C4046207!4046207!24517837     free     free     free Deprecated: Implicit conversion from float 211.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 211.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 211.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 211.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 211.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 211.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534       Stem+Cells+Dev 2014 ; 23 (12): 1355-63 Nephropedia Template TP {{tp|p=24517837|t=2014. Functional Assessment of Hematopoietic Niche Cells Derived from Human Embryonic Stem Cells |pdf=|usr=}}{{24517837}} gab.com Text Functional Assessment of Hematopoietic Niche Cells Derived from Human Embryonic Stem Cells JO: Stem Cells Dev http://www.kidney.de/pget.php?&tw=1&pmid=24517837 #FOAvip To evaluate hematopoietic niche cell populations isolated from human embryonic stem cells (hESCs), we tested the ability of hESC-derived stromal lines to support CD34+ umbilical cord blood (UCB)- and hESC-derived CD34+45+ cells in long-term culture initiating cell (LTC-IC) assays. Specifically, these hematopoietic populations were cocultured with hESC-derived mesenchymal stromal cells (hESC-MSCs) and hESC-derived endothelial cells (hESC-ECs), and then assessed for their LTC-IC potential in comparison to coculture with bone marrow (BM)-derived MSCs and the mouse stromal line M2-10B4. We found that the hESC-derived stromal lines supported LTC-ICs from UCB similar to M2-10B4 cells and better than BM-MSCs. However, none of the stromal populations supported LTC-IC from hESC-derived CD34+45+ cells. Engraftment data using the output from LTC-IC assays showed long-term repopulation (12 weeks) of NSG mice to correlate with LTC-IC support on a given stromal layer. Therefore, hESC-derived stromal lines can be used to efficiently evaluate putative hematopoietic stem/progenitor cells derived from hESCs or other cell sources. Twit Text FOAVip Functional Assessment of Hematopoietic Niche Cells Derived from Human Embryonic Stem Cells ????Stem Cells Dev http://www.kidney.de/pget.php?&tw=1&pmid=24517837 #FOAvip Twit Text # Free Paper on # # # # # LINK http://www.kidney.de/pget.php?&tw=1&pmid=24517837 #FOAvip English Wikipedia <ref>{{Cite pmid|24517837}}</ref> Functional Assessment of Hematopoietic Niche Cells Derived from Human Embryonic Stem Cells #MMPMID24517837 Ferrell PI; Hexum MK; Kopher RA; Lepley MA; Gussiaas A; Kaufman DS Stem Cells Dev 2014[Jun]; 23 (12): 1355-63 PMID24517837show ga To evaluate hematopoietic niche cell populations isolated from human embryonic stem cells (hESCs), we tested the ability of hESC-derived stromal lines to support CD34+ umbilical cord blood (UCB)- and hESC-derived CD34+45+ cells in long-term culture initiating cell (LTC-IC) assays. Specifically, these hematopoietic populations were cocultured with hESC-derived mesenchymal stromal cells (hESC-MSCs) and hESC-derived endothelial cells (hESC-ECs), and then assessed for their LTC-IC potential in comparison to coculture with bone marrow (BM)-derived MSCs and the mouse stromal line M2-10B4. We found that the hESC-derived stromal lines supported LTC-ICs from UCB similar to M2-10B4 cells and better than BM-MSCs. However, none of the stromal populations supported LTC-IC from hESC-derived CD34+45+ cells. Engraftment data using the output from LTC-IC assays showed long-term repopulation (12 weeks) of NSG mice to correlate with LTC-IC support on a given stromal layer. Therefore, hESC-derived stromal lines can be used to efficiently evaluate putative hematopoietic stem/progenitor cells derived from hESCs or other cell sources. äFunctional Assessment of Hematopoietic Niche Cells Derived from Human (epmc).pdf DeepDyve Pubget Overpricing