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10.1088/1758-5082/6/2/025007 http://scihub22266oqcxt.onion/10.1088/1758-5082/6/2/025007 C4043747!4043747!24694373     free     free     free Deprecated: Implicit conversion from float 209.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534       Biofabrication 2014 ; 6 (2): 025007 Nephropedia Template TP {{tp|p=24694373|t=2014. Generating size-controlled embryoid bodies using laser direct-write |pdf=|usr=}}{{24694373}} gab.com Text Generating size-controlled embryoid bodies using laser direct-write JO: Biofabrication http://www.kidney.de/pget.php?&tw=1&pmid=24694373 #FOAvip Embryonic stem cells (ESCs) have the potential to self-renew and differentiate into any specialized cell type. One common method to differentiate ESCs in vitro is through embryoid bodies (EBs), 3D cellular aggregates that spontaneously self-assemble and generally express markers for the three germ layers, endoderm, ectoderm, and mesoderm. It has been previously shown that both EB size and 2D colony size each influence differentiation. We hypothesized that we could control the size of the EB formed by mouse ESCs (mESCs) by using a cell printing method, laser direct-write (LDW), to control both the size of the initial printed colony and the local cell density in printed colonies. After printing mESCs at various printed colony sizes and printing densities, two-way ANOVAs indicated that EB diameter was influenced by printing density after 3 days (p = 0.0002), while there was no effect of printed colony diameter on EB diameter at the same timepoint (p = 0.74). There was no significant interaction between these two factors. Tukey?s Honestly Significant Difference test showed that high-density colonies formed significantly larger EBs, suggesting that printed mESCs quickly aggregate with nearby cells. Thus, EBs can be engineered to a desired size by controlling printing density, which will influence the design of future differentiation studies. Herein, we highlight the capacity of LDW to control the local cell density and colony size independently, at prescribed spatial locations, potentially leading to better stem cell maintenance and directed differentiation. Twit Text FOAVip Generating size-controlled embryoid bodies using laser direct-write ????Biofabrication http://www.kidney.de/pget.php?&tw=1&pmid=24694373 #FOAvip Twit Text # Free Paper on # # # # # LINK http://www.kidney.de/pget.php?&tw=1&pmid=24694373 #FOAvip English Wikipedia <ref>{{Cite pmid|24694373}}</ref> Generating size-controlled embryoid bodies using laser direct-write #MMPMID24694373 Dias A; Unser A; Xie Y; Chrisey D; Corr D Biofabrication 2014[Jun]; 6 (2): 025007 PMID24694373show ga Embryonic stem cells (ESCs) have the potential to self-renew and differentiate into any specialized cell type. One common method to differentiate ESCs in vitro is through embryoid bodies (EBs), 3D cellular aggregates that spontaneously self-assemble and generally express markers for the three germ layers, endoderm, ectoderm, and mesoderm. It has been previously shown that both EB size and 2D colony size each influence differentiation. We hypothesized that we could control the size of the EB formed by mouse ESCs (mESCs) by using a cell printing method, laser direct-write (LDW), to control both the size of the initial printed colony and the local cell density in printed colonies. After printing mESCs at various printed colony sizes and printing densities, two-way ANOVAs indicated that EB diameter was influenced by printing density after 3 days (p = 0.0002), while there was no effect of printed colony diameter on EB diameter at the same timepoint (p = 0.74). There was no significant interaction between these two factors. Tukey?s Honestly Significant Difference test showed that high-density colonies formed significantly larger EBs, suggesting that printed mESCs quickly aggregate with nearby cells. Thus, EBs can be engineered to a desired size by controlling printing density, which will influence the design of future differentiation studies. Herein, we highlight the capacity of LDW to control the local cell density and colony size independently, at prescribed spatial locations, potentially leading to better stem cell maintenance and directed differentiation. äGenerating size-controlled embryoid bodies using laser direct-write (epmc).pdf DeepDyve Pubget Overpricing