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10.1038/bcj.2014.32 http://scihub22266oqcxt.onion/10.1038/bcj.2014.32 C4042302!4042302 !24813078     free     free     free Deprecated: Implicit conversion from float 211.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 211.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 211.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 211.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 211.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 211.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 211.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 211.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 245.2 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 245.2 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 245.2 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Warning: imagejpeg(C:\Inetpub\vhosts\kidney.de\httpdocs\phplern\24813078 .jpg): Failed to open stream: No such file or directory in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 117       Blood+Cancer+J 2014 ; 4 (5 ): e210 Nephropedia Template TP {{tp|p=24813078 |t=2014. TALEN-mediated genetic tailoring as a tool to analyze the function of acquired mutations in multiple myeloma cells |pdf=|usr=}}{{24813078 }} gab.com Text TALEN-mediated genetic tailoring as a tool to analyze the function of acquired mutations in multiple myeloma cells JO: Blood Cancer J http://www.kidney.de/pget.php?&tw=1&pmid=24813078 #FOAvip Multiple myeloma (MM) is a clonal plasma cell malignancy that is initiated by a number of mutations and the process of disease progression is characterized by further acquisition of mutations. The identification and functional characterization of these myelomagenic mutations is necessary to better understand the underlying pathogenic mechanisms in this disease. Recent advancements in next-generation sequencing have made the identification of most of these mutations a reality. However, the functional characterization of these mutations has been hampered by the lack of proper and efficient tools to dissect these mutations. Here we explored the possible utility of transcription activator-like effector nuclease (TALEN) genome engineering technology to tailoring the genome of MM cells. To test this possibility, we targeted the HPRT1 gene and found that TALENs are a very robust and efficient genome-editing tool in MM cells. Using cotransfected green fluorescent protein as an enrichment marker, single-cell subclones with desirable TALEN modifications in the HPRT1 gene were obtained in as little as 3-4 weeks of time. We believe that TALENs will greatly facilitate the functional study of somatic mutations in MM as well as other cancers. Twit Text FOAVip TALEN-mediated genetic tailoring as a tool to analyze the function of acquired mutations in multiple myeloma cells ????Blood Cancer J http://www.kidney.de/pget.php?&tw=1&pmid=24813078 #FOAvip Twit Text # Free Paper on # # # # # LINK http://www.kidney.de/pget.php?&tw=1&pmid=24813078 #FOAvip English Wikipedia <ref>{{Cite pmid|24813078 }}</ref> TALEN-mediated genetic tailoring as a tool to analyze the function of acquired mutations in multiple myeloma cells #MMPMID24813078 Wu X ; Blackburn PR ; Tschumper RC ; Ekker SC ; Jelinek DF Blood Cancer J 2014[May]; 4 (5 ): e210 PMID24813078 show ga Multiple myeloma (MM) is a clonal plasma cell malignancy that is initiated by a number of mutations and the process of disease progression is characterized by further acquisition of mutations. The identification and functional characterization of these myelomagenic mutations is necessary to better understand the underlying pathogenic mechanisms in this disease. Recent advancements in next-generation sequencing have made the identification of most of these mutations a reality. However, the functional characterization of these mutations has been hampered by the lack of proper and efficient tools to dissect these mutations. Here we explored the possible utility of transcription activator-like effector nuclease (TALEN) genome engineering technology to tailoring the genome of MM cells. To test this possibility, we targeted the HPRT1 gene and found that TALENs are a very robust and efficient genome-editing tool in MM cells. Using cotransfected green fluorescent protein as an enrichment marker, single-cell subclones with desirable TALEN modifications in the HPRT1 gene were obtained in as little as 3-4 weeks of time. We believe that TALENs will greatly facilitate the functional study of somatic mutations in MM as well as other cancers. |*Mutation [MESH] |Base Sequence [MESH] |Deoxyribonucleases/*genetics/metabolism [MESH] |Female [MESH] |Gene Knockout Techniques/methods [MESH] |Gene Targeting [MESH] |Genetic Engineering [MESH] |Humans [MESH] |Hypoxanthine Phosphoribosyltransferase/genetics/metabolism [MESH] |Male [MESH] |Molecular Sequence Data [MESH] |Multiple Myeloma/*enzymology/*genetics [MESH]TALEN-mediated genetic tailoring as a tool to analyze the function of (epmc).pdf DeepDyve Pubget Overpricing