Deprecated: Implicit conversion from float 217.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534
Deprecated: Implicit conversion from float 217.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534
Deprecated: Implicit conversion from float 217.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534
Deprecated: Implicit conversion from float 217.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534
Deprecated: Implicit conversion from float 217.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534
Deprecated: Implicit conversion from float 217.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534
Deprecated: Implicit conversion from float 217.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534
Deprecated: Implicit conversion from float 217.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534
Deprecated: Implicit conversion from float 217.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534
Deprecated: Implicit conversion from float 251.2 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534
Deprecated: Implicit conversion from float 251.2 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534
Deprecated: Implicit conversion from float 251.2 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534
Warning: imagejpeg(C:\Inetpub\vhosts\kidney.de\httpdocs\phplern\24936373
.jpg): Failed to open stream: No such file or directory in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 117 Biosci+Microbiota+Food+Health
2013 ; 32
(4
): 139-48
Nephropedia Template TP
gab.com Text
Twit Text FOAVip
Twit Text #
English Wikipedia
Comparison of the accuracy and mechanism of data mining identification of the
intestinal microbiota with 7 restriction enzymes
#MMPMID24936373
Kobayashi T
; Fujiwara K
Biosci Microbiota Food Health
2013[]; 32
(4
): 139-48
PMID24936373
show ga
The intestinal microbiota compositions of 92 Japanese men were identified
following consumption of identical meals for 3 days, and collected feces were
analyzed through terminal restriction fragment length polymorphism. The obtained
operational taxonomic units (OTUs) and subjects' smoking and drinking habits,
which had 2 nominal partitions, yes or no, were analyzed by Data mining software.
Identification of subjects for each habit was successfully performed and reported
previously, but the identification accuracy was closely dependent on the species
of the applied restriction enzymes for PCR. For the sake of better selection of
enzymes and understanding the mechanisms of Data mining analysis, 516f-BslI and
516f-HaeIII, 27f-MspI and 27f-AluI and 35f-HhaI, 35f-MspI and 35f-AluI,
altogether 7 enzymes, were examined comparatively. Data mining analysis provides
a Decision tree for identification of subjects and their dividing pathways that
is produced using a limited number of OTUs, which affects the accuracy of the
results. The present report discusses not only a global comparison of accuracies
for characteristics, but also the detailed mechanisms that result in better or
worse results and the practical roles and functions of OTUs. The OTU at the 1st
step of the constructed Decision tree was the most important for any
identification, and for all cases, the combination of subsequent OTUs, which
formed later in the Decision tree, was also unignorable. Detailed dividing
pathways were traced and compared for the 7 enzymes and the future supporting
ideas were provided for better Data mining analysis of the human intestinal
microbiota.