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10.1681/ASN.2013040433 http://scihub22266oqcxt.onion/10.1681/ASN.2013040433 C4033366!4033366 !24511121     free     free     free Deprecated: Implicit conversion from float 229.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 229.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 229.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 229.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 229.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 229.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 263.2 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 263.2 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 263.2 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 263.2 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 263.2 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 263.2 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 296.79999999999995 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 296.79999999999995 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Warning: imagejpeg(C:\Inetpub\vhosts\kidney.de\httpdocs\phplern\24511121 .jpg): Failed to open stream: No such file or directory in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 117       J+Am+Soc+Nephrol 2014 ; 25 (6 ): 1171-86 Nephropedia Template TP {{tp|p=24511121 |t=2014. Thrombospondin-1 activation of signal-regulatory protein-? stimulates reactive oxygen species production and promotes renal ischemia reperfusion injury |pdf=|usr=}}{{24511121 }} gab.com Text Thrombospondin-1 activation of signal-regulatory protein- stimulates reactive oxygen species production and promotes renal ischemia reperfusion injury JO: J Am Soc Nephrol http://www.kidney.de/pget.php?&tw=1&pmid=24511121 #FOAvip Ischemia reperfusion injury (IRI) causes tissue and organ injury, in part, through alterations in tissue blood flow and the production of reactive oxygen species. The cell surface receptor signal-regulatory protein-? (SIRP-?) is expressed on inflammatory cells and suppresses phagocytosis, but the function of SIRP-? in IRI has not been determined. We reported previously that the matricellular protein thrombospondin-1 is upregulated in IRI. Here, we report a novel interaction between thrombospondin-1 and SIRP-? on nonphagocytic cells. In cell-free experiments, thrombospondin-1 bound SIRP-?. In vascular smooth muscle cells and renal tubular epithelial cells, treatment with thrombospondin-1 led to phosphorylation of SIRP-? and downstream activation of Src homology domain 2-containing phosphatase-1. Thrombospondin-1 also stimulated phosphorylation of p47(phox) (an organizer subunit for nicotinamide adenine dinucleotide phosphate (NADPH) oxidase 1/2) and increased production of superoxide, both of which were abrogated by knockdown or antibody blockade of SIRP-?. In rodent aortic rings, treatment with thrombospondin-1 increased the production of superoxide and inhibited nitric oxide-mediated vasodilation in a SIRP-?-dependent manner. Renal IRI upregulated the thrombospondin-1-SIRP-? signaling axis and was associated with increased superoxide production and cell death. A SIRP-? antibody that blocks thrombospondin-1 activation of SIRP-? mitigated the effects of renal IRI, increasing blood flow, suppressing production of reactive oxygen species, and preserving cellular architecture. A role for CD47 in SIRP-? activation in these pathways is also described. Overall, these results suggest that thrombospondin-1 binding to SIRP-? on nonphagocytic cells activates NADPH oxidase, limits vasodilation, and promotes renal IRI. Twit Text FOAVip Thrombospondin-1 activation of signal-regulatory protein- stimulates reactive oxygen species production and promotes renal ischemia reperfusion injury ????J Am Soc Nephrol http://www.kidney.de/pget.php?&tw=1&pmid=24511121 #FOAvip Twit Text # Free Paper on # # # # # LINK http://www.kidney.de/pget.php?&tw=1&pmid=24511121 #FOAvip English Wikipedia <ref>{{Cite pmid|24511121 }}</ref> Thrombospondin-1 activation of signal-regulatory protein-? stimulates reactive oxygen species production and promotes renal ischemia reperfusion injury #MMPMID24511121 Yao M ; Rogers NM ; Csányi G ; Rodriguez AI ; Ross MA ; St Croix C ; Knupp H ; Novelli EM ; Thomson AW ; Pagano PJ ; Isenberg JS J Am Soc Nephrol 2014[Jun]; 25 (6 ): 1171-86 PMID24511121 show ga Ischemia reperfusion injury (IRI) causes tissue and organ injury, in part, through alterations in tissue blood flow and the production of reactive oxygen species. The cell surface receptor signal-regulatory protein-? (SIRP-?) is expressed on inflammatory cells and suppresses phagocytosis, but the function of SIRP-? in IRI has not been determined. We reported previously that the matricellular protein thrombospondin-1 is upregulated in IRI. Here, we report a novel interaction between thrombospondin-1 and SIRP-? on nonphagocytic cells. In cell-free experiments, thrombospondin-1 bound SIRP-?. In vascular smooth muscle cells and renal tubular epithelial cells, treatment with thrombospondin-1 led to phosphorylation of SIRP-? and downstream activation of Src homology domain 2-containing phosphatase-1. Thrombospondin-1 also stimulated phosphorylation of p47(phox) (an organizer subunit for nicotinamide adenine dinucleotide phosphate (NADPH) oxidase 1/2) and increased production of superoxide, both of which were abrogated by knockdown or antibody blockade of SIRP-?. In rodent aortic rings, treatment with thrombospondin-1 increased the production of superoxide and inhibited nitric oxide-mediated vasodilation in a SIRP-?-dependent manner. Renal IRI upregulated the thrombospondin-1-SIRP-? signaling axis and was associated with increased superoxide production and cell death. A SIRP-? antibody that blocks thrombospondin-1 activation of SIRP-? mitigated the effects of renal IRI, increasing blood flow, suppressing production of reactive oxygen species, and preserving cellular architecture. A role for CD47 in SIRP-? activation in these pathways is also described. Overall, these results suggest that thrombospondin-1 binding to SIRP-? on nonphagocytic cells activates NADPH oxidase, limits vasodilation, and promotes renal IRI. |Animals [MESH] |Antigens, Differentiation/*metabolism [MESH] |Basic Helix-Loop-Helix Leucine Zipper Transcription Factors/*metabolism [MESH] |CD47 Antigen/metabolism [MESH] |Cells, Cultured [MESH] |Humans [MESH] |Kidney Diseases/*metabolism [MESH] |Male [MESH] |Mice [MESH] |Mice, Inbred C57BL [MESH] |Muscle, Smooth, Vascular/cytology/drug effects [MESH] |Nitric Oxide Donors/pharmacology [MESH] |Nitroprusside/pharmacology [MESH] |Phosphorylation/physiology [MESH] |Rats [MESH] |Rats, Sprague-Dawley [MESH] |Reactive Oxygen Species/metabolism [MESH] |Receptors, Immunologic/*metabolism [MESH] |Reperfusion Injury/*metabolism [MESH] |Signal Transduction/physiology [MESH]Thrombospondin-1 activation of signal-regulatory protein-? stimulates (epmc).pdf DeepDyve Pubget Overpricing