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2014 ; 8
(6
): 1186-97
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The role of quorum sensing signalling in EPS production and the assembly of a
sludge community into aerobic granules
#MMPMID24430488
Tan CH
; Koh KS
; Xie C
; Tay M
; Zhou Y
; Williams R
; Ng WJ
; Rice SA
; Kjelleberg S
ISME J
2014[Jun]; 8
(6
): 1186-97
PMID24430488
show ga
Quorum sensing (QS) signalling has been extensively studied in single species
populations. However, the ecological role of QS in complex, multi-species
communities, particularly in the context of community assembly, has neither been
experimentally explored nor theoretically addressed. Here, we performed a
long-term bioreactor ecology study to address the links between QS, organization
and composition of complex microbial communities. The conversion of floccular
biomass to highly structured granules was found to be non-random, but strongly
and positively correlated with N-acyl-homoserine-lactone (AHL)-mediated QS.
Specific AHLs were elevated up to 100-fold and were strongly associated with the
initiation of granulation. Similarly, the levels of particular AHLs decreased
markedly during the granular disintegration phase. Metadata analysis indicated
that granulation was accompanied by changes in extracellular polymeric substance
(EPS) production and AHL add-back studies also resulted in increased EPS
synthesis. In contrast to the commonly reported nanomolar to micromolar signal
concentrations in pure culture laboratory systems, QS signalling in the
granulation ecosystem occurred at picomolar to nanomolar concentrations of AHLs.
Given that low concentrations of AHLs quantified in this study were sufficient to
activate AHL bioreporters in situ in complex granular communities, AHL mediated
QS may be a common feature in many natural and engineered ecosystems, where it
coordinates community behaviour.