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10.1021/ml300326v http://scihub22266oqcxt.onion/10.1021/ml300326v C4027549!4027549 !24900577     free     free     free Warning: file_get_contents(https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pubmed&id=24900577 &cmd=llinks): Failed to open stream: HTTP request failed! HTTP/1.1 429 Too Many Requests in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 215       ACS+Med+Chem+Lett 2013 ; 4 (1 ): 142-5 Nephropedia Template TP {{tp|p=24900577 |t=2013. Rapid Determination of Ionization Constants (pK a) by UV Spectroscopy Using 96-Well Microtiter Plates |pdf=|usr=}}{{24900577 }} gab.com Text Rapid Determination of Ionization Constants (pK a) by UV Spectroscopy Using 96-Well Microtiter Plates JO: ACS Med Chem Lett http://www.kidney.de/pget.php?&tw=1&pmid=24900577 #FOAvip We have developed a methodology that enables for the rapid measurement of ionization constants (pK a) of series of compounds by UV spectrophotometry. This protocol, which is straightforward to set up, takes advantage of the sensitivity of UV spectroscopy and the throughput enabled by the 96-well microplate (as opposed to the use of 1 cm quartz cuvette). The compounds, in stock solutions in DMSO, are dissolved in several aqueous buffer solutions directly in the microtiter plate, allowing the simultaneous determination of the UV spectra as a function of pH. Further treatment of the data provides the pK a values in a medium-throughput manner. The pK a values of 11 new antitrypanosomal dibasic compounds were determined using this methodology. Twit Text FOAVip Rapid Determination of Ionization Constants (pK a) by UV Spectroscopy Using 96-Well Microtiter Plates ????ACS Med Chem Lett http://www.kidney.de/pget.php?&tw=1&pmid=24900577 #FOAvip Twit Text # Free Paper on # # # # # LINK http://www.kidney.de/pget.php?&tw=1&pmid=24900577 #FOAvip English Wikipedia <ref>{{Cite pmid|24900577 }}</ref> Rapid Determination of Ionization Constants (pK a) by UV Spectroscopy Using 96-Well Microtiter Plates #MMPMID24900577 Martínez CH ; Dardonville C ACS Med Chem Lett 2013[Jan]; 4 (1 ): 142-5 PMID24900577 show ga We have developed a methodology that enables for the rapid measurement of ionization constants (pK a) of series of compounds by UV spectrophotometry. This protocol, which is straightforward to set up, takes advantage of the sensitivity of UV spectroscopy and the throughput enabled by the 96-well microplate (as opposed to the use of 1 cm quartz cuvette). The compounds, in stock solutions in DMSO, are dissolved in several aqueous buffer solutions directly in the microtiter plate, allowing the simultaneous determination of the UV spectra as a function of pH. Further treatment of the data provides the pK a values in a medium-throughput manner. The pK a values of 11 new antitrypanosomal dibasic compounds were determined using this methodology. ?Rapid Determination of Ionization Constants (pK a) by UV Spectroscopy (epmc).pdf DeepDyve Pubget Overpricing