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10.1089/ten.tec.2013.0335 http://scihub22266oqcxt.onion/10.1089/ten.tec.2013.0335 C4024844!4024844!24188635     free     free     free Deprecated: Implicit conversion from float 233.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 233.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534       Tissue+Eng+Part+C+Methods 2014 ; 20 (6): 473-84 Nephropedia Template TP {{tp|p=24188635|t=2014. Design and Fabrication of Human Skin by Three-Dimensional Bioprinting |pdf=|usr=}}{{24188635}} gab.com Text Design and Fabrication of Human Skin by Three-Dimensional Bioprinting JO: Tissue Eng Part C Methods http://www.kidney.de/pget.php?&tw=1&pmid=24188635 #FOAvip Three-dimensional (3D) bioprinting, a flexible automated on-demand platform for the free-form fabrication of complex living architectures, is a novel approach for the design and engineering of human organs and tissues. Here, we demonstrate the potential of 3D bioprinting for tissue engineering using human skin as a prototypical example. Keratinocytes and fibroblasts were used as constituent cells to represent the epidermis and dermis, and collagen was used to represent the dermal matrix of the skin. Preliminary studies were conducted to optimize printing parameters for maximum cell viability as well as for the optimization of cell densities in the epidermis and dermis to mimic physiologically relevant attributes of human skin. Printed 3D constructs were cultured in submerged media conditions followed by exposure of the epidermal layer to the air?liquid interface to promote maturation and stratification. Histology and immunofluorescence characterization demonstrated that 3D printed skin tissue was morphologically and biologically representative of in vivo human skin tissue. In comparison with traditional methods for skin engineering, 3D bioprinting offers several advantages in terms of shape- and form retention, flexibility, reproducibility, and high culture throughput. It has a broad range of applications in transdermal and topical formulation discovery, dermal toxicity studies, and in designing autologous grafts for wound healing. The proof-of-concept studies presented here can be further extended for enhancing the complexity of the skin model via the incorporation of secondary and adnexal structures or the inclusion of diseased cells to serve as a model for studying the pathophysiology of skin diseases. Twit Text FOAVip Design and Fabrication of Human Skin by Three-Dimensional Bioprinting ????Tissue Eng Part C Methods http://www.kidney.de/pget.php?&tw=1&pmid=24188635 #FOAvip Twit Text # Free Paper on # # # # # LINK http://www.kidney.de/pget.php?&tw=1&pmid=24188635 #FOAvip English Wikipedia <ref>{{Cite pmid|24188635}}</ref> Design and Fabrication of Human Skin by Three-Dimensional Bioprinting #MMPMID24188635 Lee V; Singh G; Trasatti JP; Bjornsson C; Xu X; Tran TN; Yoo SS; Dai G; Karande P Tissue Eng Part C Methods 2014[Jun]; 20 (6): 473-84 PMID24188635show ga Three-dimensional (3D) bioprinting, a flexible automated on-demand platform for the free-form fabrication of complex living architectures, is a novel approach for the design and engineering of human organs and tissues. Here, we demonstrate the potential of 3D bioprinting for tissue engineering using human skin as a prototypical example. Keratinocytes and fibroblasts were used as constituent cells to represent the epidermis and dermis, and collagen was used to represent the dermal matrix of the skin. Preliminary studies were conducted to optimize printing parameters for maximum cell viability as well as for the optimization of cell densities in the epidermis and dermis to mimic physiologically relevant attributes of human skin. Printed 3D constructs were cultured in submerged media conditions followed by exposure of the epidermal layer to the air?liquid interface to promote maturation and stratification. Histology and immunofluorescence characterization demonstrated that 3D printed skin tissue was morphologically and biologically representative of in vivo human skin tissue. In comparison with traditional methods for skin engineering, 3D bioprinting offers several advantages in terms of shape- and form retention, flexibility, reproducibility, and high culture throughput. It has a broad range of applications in transdermal and topical formulation discovery, dermal toxicity studies, and in designing autologous grafts for wound healing. The proof-of-concept studies presented here can be further extended for enhancing the complexity of the skin model via the incorporation of secondary and adnexal structures or the inclusion of diseased cells to serve as a model for studying the pathophysiology of skin diseases. äDesign and Fabrication of Human Skin by Three-Dimensional Bioprinting (epmc).pdf DeepDyve Pubget Overpricing