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2014 ; 21
(6
): 716-23
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Expression of notch family proteins in placentas from patients with early-onset
severe preeclampsia
#MMPMID24336671
Zhao WX
; Huang TT
; Jiang M
; Feng R
; Lin JH
Reprod Sci
2014[Jun]; 21
(6
): 716-23
PMID24336671
show ga
OBJECTIVES: This study is aimed to identify the expression of Notch family
proteins in placentas from patients with early-onset severe preeclampsia. STUDY
DESIGN: The expression of Notch family proteins in placentas was investigated by
immunohistochemistry, Western blotting, and real-time reverse
transcription-polymerase chain reaction (RT-PCR). RESULTS: The profile of
distribution of all Notch family proteins in placentas from patients with
early-onset severe preeclampsia is similar to that in normal placentas. All Notch
family proteins are expressed in placental trophoblasts. Moreover, Notch1 and
Jagged1 (Jag1) are detected in placental endothelial cells. Real-time RT-PCR
showed that messenger RNA levels of Notch2 and Delta-like4 (Dll4) in placentas
from patients with early-onset severe preeclampsia are lower than that of normal
placentas. Western blotting showed a significant increase in Notch3 expression
and a significant decrease in Notch2 expression in placentas from patients with
early-onset severe preeclampsia relative to those in normal placentas.
CONCLUSION: The results suggest that Notch2 and Notch3 may play some roles in the
pathogenesis of preeclampsia.