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10.1007/s10439-014-0992-x http://scihub22266oqcxt.onion/10.1007/s10439-014-0992-x C4011989!4011989!24577875     free     free     free Warning: file_get_contents(https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pubmed&id=24577875&cmd=llinks): Failed to open stream: HTTP request failed! HTTP/1.1 500 Internal Server Error in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 215 Deprecated: Implicit conversion from float 209.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 209.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 209.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534       Ann+Biomed+Eng 2014 ; 42 (6): 1148-57 Nephropedia Template TP {{tp|p=24577875|t=2014. Role of the Basement Membrane in Regulation of Cardiac Electrical Properties |pdf=|usr=}}{{24577875}} gab.com Text Role of the Basement Membrane in Regulation of Cardiac Electrical Properties JO: Ann Biomed Eng http://www.kidney.de/pget.php?&tw=1&pmid=24577875 #FOAvip In the heart muscle, each adult cardiomyocyte is enclosed by a basement membrane (BM). This innermost extracellular matrix is a layered assembly of laminin, collagen IV, glycoproteins, and proteoglycans. In this study, the role of the BM network in regulation of the electrical properties of neonatal cardiomyocytes (NCMs) cultured on an aligned collagen I gel was investigated using a multielectrode array (MEA). A laminin antibody was added to the culture medium for 48?120 h to conjugate newly secreted laminin. Then, morphology of the NCMs on an MEA was monitored using a phase contrast microscope, and the BM network that was immunocytostained for laminin was imaged using a fluorescence microscope. When the BM laminin was absent in this culture model, dramatic changes in NCM morphology were observed. Simultaneously, the MEA-recorded cardiac field potential showed changes compared to that from the control groups: The period of contraction shortened to 1/2 of that from the control groups, and the waveform of the calcium influx shifted from a flat plateau to a peak-like waveform, indicating that the electrical properties of the NCMs were closely related to the components and distribution of the BM network. Twit Text FOAVip Role of the Basement Membrane in Regulation of Cardiac Electrical Properties ????Ann Biomed Eng http://www.kidney.de/pget.php?&tw=1&pmid=24577875 #FOAvip Twit Text # Free Paper on # # # # # LINK http://www.kidney.de/pget.php?&tw=1&pmid=24577875 #FOAvip English Wikipedia <ref>{{Cite pmid|24577875}}</ref> Role of the Basement Membrane in Regulation of Cardiac Electrical Properties #MMPMID24577875 Yang H; Borg TK; Wang Z; Ma Z; Gao BZ Ann Biomed Eng 2014[Jun]; 42 (6): 1148-57 PMID24577875show ga In the heart muscle, each adult cardiomyocyte is enclosed by a basement membrane (BM). This innermost extracellular matrix is a layered assembly of laminin, collagen IV, glycoproteins, and proteoglycans. In this study, the role of the BM network in regulation of the electrical properties of neonatal cardiomyocytes (NCMs) cultured on an aligned collagen I gel was investigated using a multielectrode array (MEA). A laminin antibody was added to the culture medium for 48?120 h to conjugate newly secreted laminin. Then, morphology of the NCMs on an MEA was monitored using a phase contrast microscope, and the BM network that was immunocytostained for laminin was imaged using a fluorescence microscope. When the BM laminin was absent in this culture model, dramatic changes in NCM morphology were observed. Simultaneously, the MEA-recorded cardiac field potential showed changes compared to that from the control groups: The period of contraction shortened to 1/2 of that from the control groups, and the waveform of the calcium influx shifted from a flat plateau to a peak-like waveform, indicating that the electrical properties of the NCMs were closely related to the components and distribution of the BM network. äRole of the Basement Membrane in Regulation of Cardiac Electrical Prop(epmc).pdf DeepDyve Pubget Overpricing