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10.1016/j.bpj.2014.03.009 http://scihub22266oqcxt.onion/10.1016/j.bpj.2014.03.009 C4008835!4008835!24739157     free     free     free Deprecated: Implicit conversion from float 213.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 213.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534       Biophys+J 2014 ; 106 (8): 1578-87 Nephropedia Template TP {{tp|p=24739157|t=2014. Measuring Microtubule Polarity in Spindles with Second-Harmonic Generation |pdf=|usr=}}{{24739157}} gab.com Text Measuring Microtubule Polarity in Spindles with Second-Harmonic Generation JO: Biophys J http://www.kidney.de/pget.php?&tw=1&pmid=24739157 #FOAvip The spatial organization of microtubule polarity, and the interplay between microtubule polarity and protein localization, is thought to be crucial for spindle assembly, anaphase, and cytokinesis, but these phenomena remain poorly understood, in part due to the difficulty of measuring microtubule polarity in spindles. We develop and implement a method to nonperturbatively and quantitatively measure microtubule polarity throughout spindles using a combination of second-harmonic generation and two-photon fluorescence. We validate this method using computer simulations and by comparison to structural data on spindles obtained from electron tomography and laser ablation. This method should provide a powerful tool for studying spindle organization and function, and may be applicable for investigating microtubule polarity in other systems. Twit Text FOAVip Measuring Microtubule Polarity in Spindles with Second-Harmonic Generation ????Biophys J http://www.kidney.de/pget.php?&tw=1&pmid=24739157 #FOAvip Twit Text # Free Paper on # # # # # LINK http://www.kidney.de/pget.php?&tw=1&pmid=24739157 #FOAvip English Wikipedia <ref>{{Cite pmid|24739157}}</ref> Measuring Microtubule Polarity in Spindles with Second-Harmonic Generation #MMPMID24739157 Yu CH; Langowitz N; Wu HY; Farhadifar R; Brugues J; Yoo T; Needleman D Biophys J 2014[Apr]; 106 (8): 1578-87 PMID24739157show ga The spatial organization of microtubule polarity, and the interplay between microtubule polarity and protein localization, is thought to be crucial for spindle assembly, anaphase, and cytokinesis, but these phenomena remain poorly understood, in part due to the difficulty of measuring microtubule polarity in spindles. We develop and implement a method to nonperturbatively and quantitatively measure microtubule polarity throughout spindles using a combination of second-harmonic generation and two-photon fluorescence. We validate this method using computer simulations and by comparison to structural data on spindles obtained from electron tomography and laser ablation. This method should provide a powerful tool for studying spindle organization and function, and may be applicable for investigating microtubule polarity in other systems. äMeasuring Microtubule Polarity in Spindles with Second-Harmonic Genera(epmc).pdf DeepDyve Pubget Overpricing