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10.1159/000353199 http://scihub22266oqcxt.onion/10.1159/000353199 C4007268!4007268!23774784     free     free     free Deprecated: Implicit conversion from float 217.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 217.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 217.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 217.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 217.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 217.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 217.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534       Nephron+Physiol 2012 ; 122 (ä): 19-27 Nephropedia Template TP {{tp|p=23774784|t=2012. Variations of Dietary Salt and Fluid Modulate Calcium and Magnesium Transport in Renal Distal Tubule |pdf=|usr=}}{{23774784}} gab.com Text Variations of Dietary Salt and Fluid Modulate Calcium and Magnesium Transport in Renal Distal Tubule JO: Nephron Physiol http://www.kidney.de/pget.php?&tw=1&pmid=23774784 #FOAvip Background: The renal distal tubule serves as the fine tuning of renal epithelial calcium transport. Dietary intake of salt and fluid varies day to day and the kidney adapts accordingly to maintain the homeostasis. The alternations in salt and fluid balance affect calcium and magnesium transport in the distal tubule, but the mechanisms are not fully understood. Methods: Sprague-Dawley rats were grouped into high salt, low salt and dehydration treatment. Daily intake, water consumption and urine output were recorded. At the end of experiment, blood and urine samples were collected for hormonal and biochemical testes. Genetic analysis, immunoblotting, and immunofluorescence studies were then performed to assess the alterations of calcium and magnesium transport-related molecules. Results: High salt treatment increased urinary sodium, calcium and magnesium excretion. Low salt treatment and dehydration were associated with decreased urinary excretion of all electrolytes. High salt treatment was associated with increased intact parathyroid hormone levels. Significant increase in gene expression of TRPV5, TRPV6, calbindin-D28k and TRPM6 was found during high salt treatment while low salt and dehydration diminished the expression. These findings were confirmed with immunofluorescence studies. High salt and low salt intake or dehydration did not cause any significant changes in WNK1, WNK3 and WNK4. Conclusions: Alternations in salt and water intake affect renal calcium and magnesium handling. High salt intake increases distal delivery of the divalent cations which upregulates distal tubule calcium and magnesium transport molecules, while the opposite effects are associated with low salt intake or dehydration. Twit Text FOAVip Variations of Dietary Salt and Fluid Modulate Calcium and Magnesium Transport in Renal Distal Tubule ????Nephron Physiol http://www.kidney.de/pget.php?&tw=1&pmid=23774784 #FOAvip Twit Text # Free Paper on # # # # # LINK http://www.kidney.de/pget.php?&tw=1&pmid=23774784 #FOAvip English Wikipedia <ref>{{Cite pmid|23774784}}</ref> Variations of Dietary Salt and Fluid Modulate Calcium and Magnesium Transport in Renal Distal Tubule #MMPMID23774784 Lee CT; Lien YHH; Lai LW; Ng HY; Chiou TTY; Chen HC Nephron Physiol 2012[]; 122 (ä): 19-27 PMID23774784show ga Background: The renal distal tubule serves as the fine tuning of renal epithelial calcium transport. Dietary intake of salt and fluid varies day to day and the kidney adapts accordingly to maintain the homeostasis. The alternations in salt and fluid balance affect calcium and magnesium transport in the distal tubule, but the mechanisms are not fully understood. Methods: Sprague-Dawley rats were grouped into high salt, low salt and dehydration treatment. Daily intake, water consumption and urine output were recorded. At the end of experiment, blood and urine samples were collected for hormonal and biochemical testes. Genetic analysis, immunoblotting, and immunofluorescence studies were then performed to assess the alterations of calcium and magnesium transport-related molecules. Results: High salt treatment increased urinary sodium, calcium and magnesium excretion. Low salt treatment and dehydration were associated with decreased urinary excretion of all electrolytes. High salt treatment was associated with increased intact parathyroid hormone levels. Significant increase in gene expression of TRPV5, TRPV6, calbindin-D28k and TRPM6 was found during high salt treatment while low salt and dehydration diminished the expression. These findings were confirmed with immunofluorescence studies. High salt and low salt intake or dehydration did not cause any significant changes in WNK1, WNK3 and WNK4. Conclusions: Alternations in salt and water intake affect renal calcium and magnesium handling. High salt intake increases distal delivery of the divalent cations which upregulates distal tubule calcium and magnesium transport molecules, while the opposite effects are associated with low salt intake or dehydration. äVariations of Dietary Salt and Fluid Modulate Calcium and Magnesium Tr(epmc).pdf DeepDyve Pubget Overpricing