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10.1136/gutjnl-2013-305962 http://scihub22266oqcxt.onion/10.1136/gutjnl-2013-305962 C4006344!4006344!24173292     free     free     free Deprecated: Implicit conversion from float 211.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 211.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 211.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 211.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 211.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 211.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534       Gut 2014 ; 63 (8): 1333-44 Nephropedia Template TP {{tp|p=24173292|t=2014. Myofibroblastic Cells Function as Progenitors to Regenerate Murine Livers after Partial Hepatectomy |pdf=|usr=}}{{24173292}} gab.com Text Myofibroblastic Cells Function as Progenitors to Regenerate Murine Livers after Partial Hepatectomy JO: Gut http://www.kidney.de/pget.php?&tw=1&pmid=24173292 #FOAvip Objective: Smoothened (SMO), a co-receptor of the Hedgehog (Hh) pathway, promotes fibrogenic repair of chronic liver injury. We investigated the roles of SMO+ myofibroblasts (MF) in liver regeneration by conditional deletion of SMO in ?SMA+ cells after partial hepatectomy (PH). Design: ?SMA-Cre-ERT2×SMO/flox mice were treated with vehicle (Veh) or tamoxifen (TMX), and sacrificed 24 to 96 hrs post-PH. Regenerating livers were analyzed for proliferation, progenitors, and fibrosis by qRT-PCR and quantitative-IHC. Results were normalized to liver-segments resected at PH. For lineage-tracing studies, ?SMA-Cre-ERT2×ROSA-Stop-flox-YFP mice were treated with Veh or TMX; livers were stained for YFP, and hepatocytes isolated 48 and 72 hrs post-PH were analysed for YFP by FACS. Results: Post-PH, Veh-?SMA-SMO mice increased expression of Hh-genes, transiently accumulated MF, fibrosis, and liver progenitors, and ultimately exhibited proliferation of hepatocytes and cholangiocytes. In contrast, TMX-?SMA-SMO mice showed loss of whole liver SMO expression, repression of Hh-genes, enhanced accumulation of quiescent HSC but reduced accumulation of MF, fibrosis, and progenitors, as well as inhibition of hepatocyte and cholangiocyte proliferation, and reduced recovery of liver weight. In TMX-?SMA-YFP mice, many progenitors, cholangiocytes, and up to 25% of hepatocytes were YFP+ by 48-72 h after PH, indicating that liver epithelial cells were derived from ?SMA-YFP+cells. Conclusion: Hedgehog signaling promotes transition of quiescent hepatic stellate cells to fibrogenic MF, some of which become progenitors that regenerate the liver epithelial compartment after PH. Hence, scarring is a component of successful liver regeneration. Twit Text FOAVip Myofibroblastic Cells Function as Progenitors to Regenerate Murine Livers after Partial Hepatectomy ????Gut http://www.kidney.de/pget.php?&tw=1&pmid=24173292 #FOAvip Twit Text # Free Paper on # # # # # LINK http://www.kidney.de/pget.php?&tw=1&pmid=24173292 #FOAvip English Wikipedia <ref>{{Cite pmid|24173292}}</ref> Myofibroblastic Cells Function as Progenitors to Regenerate Murine Livers after Partial Hepatectomy #MMPMID24173292 Swiderska-Syn M; Syn W; Xie G; Krüger L; Machado M; Karaca G; Michelotti G; Choi S; Premont R; Diehl A Gut 2014[Aug]; 63 (8): 1333-44 PMID24173292show ga Objective: Smoothened (SMO), a co-receptor of the Hedgehog (Hh) pathway, promotes fibrogenic repair of chronic liver injury. We investigated the roles of SMO+ myofibroblasts (MF) in liver regeneration by conditional deletion of SMO in ?SMA+ cells after partial hepatectomy (PH). Design: ?SMA-Cre-ERT2×SMO/flox mice were treated with vehicle (Veh) or tamoxifen (TMX), and sacrificed 24 to 96 hrs post-PH. Regenerating livers were analyzed for proliferation, progenitors, and fibrosis by qRT-PCR and quantitative-IHC. Results were normalized to liver-segments resected at PH. For lineage-tracing studies, ?SMA-Cre-ERT2×ROSA-Stop-flox-YFP mice were treated with Veh or TMX; livers were stained for YFP, and hepatocytes isolated 48 and 72 hrs post-PH were analysed for YFP by FACS. Results: Post-PH, Veh-?SMA-SMO mice increased expression of Hh-genes, transiently accumulated MF, fibrosis, and liver progenitors, and ultimately exhibited proliferation of hepatocytes and cholangiocytes. In contrast, TMX-?SMA-SMO mice showed loss of whole liver SMO expression, repression of Hh-genes, enhanced accumulation of quiescent HSC but reduced accumulation of MF, fibrosis, and progenitors, as well as inhibition of hepatocyte and cholangiocyte proliferation, and reduced recovery of liver weight. In TMX-?SMA-YFP mice, many progenitors, cholangiocytes, and up to 25% of hepatocytes were YFP+ by 48-72 h after PH, indicating that liver epithelial cells were derived from ?SMA-YFP+cells. Conclusion: Hedgehog signaling promotes transition of quiescent hepatic stellate cells to fibrogenic MF, some of which become progenitors that regenerate the liver epithelial compartment after PH. Hence, scarring is a component of successful liver regeneration. äMyofibroblastic Cells Function as Progenitors to Regenerate Murine Liv(epmc).pdf DeepDyve Pubget Overpricing