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10.1002/0471143030.cb0411s59

http://scihub22266oqcxt.onion/10.1002/0471143030.cb0411s59
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C4004770!4004770!23728746
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suck abstract from ncbi


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pmid23728746      Curr+Protoc+Cell+Biol 2013 ; 0 4 (ä): Unit-4.1124
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  • Two-Photon Excitation Microscopy for the Study of Living Cells and Tissues #MMPMID23728746
  • Benninger RK; Piston DW
  • Curr Protoc Cell Biol 2013[Jun]; 0 4 (ä): Unit-4.1124 PMID23728746show ga
  • Two-photon excitation microscopy is an alternative to confocal microscopy that provides advantages for three-dimensional and deep tissue imaging. This unit will describe the basic physical principles behind two-photon excitation and discuss the advantages and limitations of its use in laser-scanning microscopy. The principal advantages of two-photon microscopy are reduced phototoxicity, increased imaging depth, and the ability to initiate highly localized photochemistry in thick samples. Practical considerations for the application of two-photon microscopy will then be discussed, including recent technological advances. This unit will conclude with some recent applications of two-photon microscopy that highlight the key advantages over confocal microscopy and the types of experiments which would benefit most from its application.
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