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10.1021/bi4017022 http://scihub22266oqcxt.onion/10.1021/bi4017022 C4004221!4004221!24694349     free     free     free Warning: file_get_contents(https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pubmed&id=24694349&cmd=llinks): Failed to open stream: HTTP request failed! HTTP/1.1 429 Too Many Requests in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 215 Deprecated: Implicit conversion from float 211.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 211.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 211.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 211.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 211.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534       Biochemistry 2014 ; 53 (14): 2221-31 Nephropedia Template TP {{tp|p=24694349|t=2014. Entropy-Driven One-Step Formation of Phi29 pRNA 3WJ from Three RNA Fragments |pdf=|usr=}}{{24694349}} gab.com Text Entropy-Driven One-Step Formation of Phi29 pRNA 3WJ from Three RNA Fragments JO: Biochemistry http://www.kidney.de/pget.php?&tw=1&pmid=24694349 #FOAvip The emerging field of RNA nanotechnology necessitates creation of functional RNA nanoparticles but has been limited by particle instability. It has been shown that the three-way junction of bacteriophage phi29 motor pRNA has unusual stability and can self-assemble from three fragments with high efficiency. It is generally believed that RNA and DNA folding is energy landscape-dependent, and the folding of RNA is driven by enthalpy. Here we examine the thermodynamic characteristics of the 3WJ components as 2?-fluoro RNA, DNA, and RNA. It was seen that the three fragments existed either in 3WJ complex or as monomers, with the intermediate of dimers almost undetectable. It seems that the three fragments can lead to the formation of the 3WJ complex efficiently within a rapid time. A low dissociation constant (apparent KD) of 11.4 nM was determined for RNA, inclusion of 2?-F pyrimidines strengthened the KD to 4.5 nM, and substitution of DNA weakened it to 47.7 nM. The ?G°37, were ?36, ?28, and ?15 kcal/mol for 3WJ2?-F, 3WJRNA, and 3WJDNA, respectively. It is found that the formation of the three-component complex was governed by entropy, instead of enthalpy, as usually found in RNA complexes. Twit Text FOAVip Entropy-Driven One-Step Formation of Phi29 pRNA 3WJ from Three RNA Fragments ????Biochemistry http://www.kidney.de/pget.php?&tw=1&pmid=24694349 #FOAvip Twit Text # Free Paper on # # # # # LINK http://www.kidney.de/pget.php?&tw=1&pmid=24694349 #FOAvip English Wikipedia <ref>{{Cite pmid|24694349}}</ref> Entropy-Driven One-Step Formation of Phi29 pRNA 3WJ from Three RNA Fragments #MMPMID24694349 Binzel D; Khisamutdinov EF; Guo P Biochemistry 2014[Apr]; 53 (14): 2221-31 PMID24694349show ga The emerging field of RNA nanotechnology necessitates creation of functional RNA nanoparticles but has been limited by particle instability. It has been shown that the three-way junction of bacteriophage phi29 motor pRNA has unusual stability and can self-assemble from three fragments with high efficiency. It is generally believed that RNA and DNA folding is energy landscape-dependent, and the folding of RNA is driven by enthalpy. Here we examine the thermodynamic characteristics of the 3WJ components as 2?-fluoro RNA, DNA, and RNA. It was seen that the three fragments existed either in 3WJ complex or as monomers, with the intermediate of dimers almost undetectable. It seems that the three fragments can lead to the formation of the 3WJ complex efficiently within a rapid time. A low dissociation constant (apparent KD) of 11.4 nM was determined for RNA, inclusion of 2?-F pyrimidines strengthened the KD to 4.5 nM, and substitution of DNA weakened it to 47.7 nM. The ?G°37, were ?36, ?28, and ?15 kcal/mol for 3WJ2?-F, 3WJRNA, and 3WJDNA, respectively. It is found that the formation of the three-component complex was governed by entropy, instead of enthalpy, as usually found in RNA complexes. äEntropy-Driven One-Step Formation of Phi29 pRNA 3WJ from Three RNA Fra(epmc).pdf DeepDyve Pubget Overpricing