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10.1089/humc.2012.244 http://scihub22266oqcxt.onion/10.1089/humc.2012.244 C4003467!4003467!23786330     free     free     free Deprecated: Implicit conversion from float 209.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 209.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 209.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 209.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 209.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 209.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 243.2 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 243.2 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 243.2 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534       Hum+Gene+Ther+Clin+Dev 2013 ; 24 (2): 77-85 Nephropedia Template TP {{tp|p=23786330|t=2013. Comparison of Insulators and Promoters for Expression of the Wiskott?Aldrich Syndrome Protein Using Lentiviral Vectors |pdf=|usr=}}{{23786330}} gab.com Text Comparison of Insulators and Promoters for Expression of the Wiskott Aldrich Syndrome Protein Using Lentiviral Vectors JO: Hum Gene Ther Clin Dev http://www.kidney.de/pget.php?&tw=1&pmid=23786330 #FOAvip Gene therapy for the treatment of Wiskott?Aldrich syndrome (WAS) presents an alternative to the current use of allogeneic bone marrow transplantation. We describe the development of a self-inactivating lentiviral vector containing chromatin insulators for treatment of WAS and compare a gammaretroviral (MND), human cellular (EF1?), and the human WASp gene promoter for expression patterns in vivo during murine hematopoiesis using the green fluorescent protein (GFP) marker. Compared with the EF1? and the WASp promoters, expression from the MND promoter in mouse transplant recipients was much higher in all lineages examined. Importantly, there was sustained expression in the platelets of secondary recipient animals, necessary to correct the thrombocytopenia defect in WAS patients. Analysis of WAS protein expression in transduced human EBV-immortalized B-cells and transduced patient peripheral blood mononuclear cells also demonstrated stronger expression per copy from the MND promoter compared with the other promoters. In addition, when analyzed in an LM02 activation assay, the addition of an insulator to MND-promoter-containing constructs reduced transactivation of the LM02 gene. We propose a clinical trial design in which cytokine-mobilized, autologous, transduced CD34+ cells are administered after myelosuppression. Twit Text FOAVip Comparison of Insulators and Promoters for Expression of the Wiskott Aldrich Syndrome Protein Using Lentiviral Vectors ????Hum Gene Ther Clin Dev http://www.kidney.de/pget.php?&tw=1&pmid=23786330 #FOAvip Twit Text # Free Paper on # # # # # LINK http://www.kidney.de/pget.php?&tw=1&pmid=23786330 #FOAvip English Wikipedia <ref>{{Cite pmid|23786330}}</ref> Comparison of Insulators and Promoters for Expression of the Wiskott?Aldrich Syndrome Protein Using Lentiviral Vectors #MMPMID23786330 Koldej RM; Carney G; Wielgosz MM; Zhou S; Zhan J; Sorrentino BP; Nienhuis AW Hum Gene Ther Clin Dev 2013[Jun]; 24 (2): 77-85 PMID23786330show ga Gene therapy for the treatment of Wiskott?Aldrich syndrome (WAS) presents an alternative to the current use of allogeneic bone marrow transplantation. We describe the development of a self-inactivating lentiviral vector containing chromatin insulators for treatment of WAS and compare a gammaretroviral (MND), human cellular (EF1?), and the human WASp gene promoter for expression patterns in vivo during murine hematopoiesis using the green fluorescent protein (GFP) marker. Compared with the EF1? and the WASp promoters, expression from the MND promoter in mouse transplant recipients was much higher in all lineages examined. Importantly, there was sustained expression in the platelets of secondary recipient animals, necessary to correct the thrombocytopenia defect in WAS patients. Analysis of WAS protein expression in transduced human EBV-immortalized B-cells and transduced patient peripheral blood mononuclear cells also demonstrated stronger expression per copy from the MND promoter compared with the other promoters. In addition, when analyzed in an LM02 activation assay, the addition of an insulator to MND-promoter-containing constructs reduced transactivation of the LM02 gene. We propose a clinical trial design in which cytokine-mobilized, autologous, transduced CD34+ cells are administered after myelosuppression. äComparison of Insulators and Promoters for Expression of the Wiskott?A(epmc).pdf DeepDyve Pubget Overpricing