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10.1016/j.ymeth.2013.10.009 http://scihub22266oqcxt.onion/10.1016/j.ymeth.2013.10.009 C3989378!3989378!24184187     free     free     free       Methods 2014 ; 66 (3): 454-65 Nephropedia Template TP {{tp|p=24184187|t=2014. Morpholinos: studying gene function in the chick |pdf=|usr=}}{{24184187}} gab.com Text Morpholinos: studying gene function in the chick JO: Methods http://www.kidney.de/pget.php?&tw=1&pmid=24184187 #FOAvip The use of morpholinos for perturbing gene function in the chick, Gallus gallus, has led to many important discoveries in developmental biology. This technology makes use of in vivo electroporation, which allows gain and loss of function in a temporally, and spatially controlled manner. Using this method, morpholinos can be transfected into embryonic tissues from early to late developmental stages. In this article, we describe the methods currently used in our laboratory to knock down gene function using morpholinos in vivo. We also detail how morpholinos are used to provide consistency of the results, and describe two protocols to visualise the morpholino after electroporation. In addition, we provide guidance on avoiding potential pitfalls, and suggestions for troubleshooting solutions. These revised techniques provide a practical starting point for investigating gene function in the chick. Twit Text FOAVip Morpholinos: studying gene function in the chick ????Methods http://www.kidney.de/pget.php?&tw=1&pmid=24184187 #FOAvip Twit Text # Free Paper on # # # # # LINK http://www.kidney.de/pget.php?&tw=1&pmid=24184187 #FOAvip English Wikipedia <ref>{{Cite pmid|24184187}}</ref> Morpholinos: studying gene function in the chick #MMPMID24184187 Norris A; Streit A Methods 2014[Apr]; 66 (3): 454-65 PMID24184187show ga The use of morpholinos for perturbing gene function in the chick, Gallus gallus, has led to many important discoveries in developmental biology. This technology makes use of in vivo electroporation, which allows gain and loss of function in a temporally, and spatially controlled manner. Using this method, morpholinos can be transfected into embryonic tissues from early to late developmental stages. In this article, we describe the methods currently used in our laboratory to knock down gene function using morpholinos in vivo. We also detail how morpholinos are used to provide consistency of the results, and describe two protocols to visualise the morpholino after electroporation. In addition, we provide guidance on avoiding potential pitfalls, and suggestions for troubleshooting solutions. These revised techniques provide a practical starting point for investigating gene function in the chick. äMorpholinos: studying gene function in the chick (epmc).pdf DeepDyve Pubget Overpricing