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10.1016/j.bios.2014.01.018 http://scihub22266oqcxt.onion/10.1016/j.bios.2014.01.018 C3977152!3977152 !24549115     free     free     free Deprecated: Implicit conversion from float 213.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 213.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 213.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 213.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 213.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 213.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 213.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 213.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Warning: imagejpeg(C:\Inetpub\vhosts\kidney.de\httpdocs\phplern\24549115 .jpg): Failed to open stream: No such file or directory in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 117       Biosens+Bioelectron 2014 ; 56 (ä): 359-67 Nephropedia Template TP {{tp|p=24549115 |t=2014. Surface plasmon resonance fiber sensor for real-time and label-free monitoring of cellular behavior |pdf=|usr=}}{{24549115 }} gab.com Text Surface plasmon resonance fiber sensor for real-time and label-free monitoring of cellular behavior JO: Biosens Bioelectron http://www.kidney.de/pget.php?&tw=1&pmid=24549115 #FOAvip This paper reports on the application of an optical fiber biosensor for real-time analysis of cellular behavior. Our findings illustrate that a fiber sensor fabricated from a traditional telecommunication fiber can be integrated into conventional cell culture equipment and used for real-time and label-free monitoring of cellular responses to chemical stimuli. The sensing mechanism used for the measurement of cellular responses is based on the excitation of surface plasmon resonance (SPR) on the surface of the optical fiber. In this proof of concept study, the sensor was utilized to investigate the influence of a number of different stimuli on cells-we tested the effects of trypsin, serum and sodium azide. These stimuli induced detachment of cells from the sensor surface, uptake of serum and inhibition of cellular metabolism, accordingly. The effects of different stimuli were confirmed with alamar blue assay, phase contrast and fluorescence microscopy. The results indicated that the fiber biosensor can be successfully utilized for real-time and label-free monitoring of cellular response in the first 30 min following the introduction of a stimulus. Furthermore, we demonstrated that the optical fiber biosensors can be easily regenerated for repeated use, proving this platform as a versatile and cost-effective sensing tool. Twit Text FOAVip Surface plasmon resonance fiber sensor for real-time and label-free monitoring of cellular behavior ????Biosens Bioelectron http://www.kidney.de/pget.php?&tw=1&pmid=24549115 #FOAvip Twit Text # Free Paper on # # # # # LINK http://www.kidney.de/pget.php?&tw=1&pmid=24549115 #FOAvip English Wikipedia <ref>{{Cite pmid|24549115 }}</ref> Surface plasmon resonance fiber sensor for real-time and label-free monitoring of cellular behavior #MMPMID24549115 Shevchenko Y ; Camci-Unal G ; Cuttica DF ; Dokmeci MR ; Albert J ; Khademhosseini A Biosens Bioelectron 2014[Jun]; 56 (ä): 359-67 PMID24549115 show ga This paper reports on the application of an optical fiber biosensor for real-time analysis of cellular behavior. Our findings illustrate that a fiber sensor fabricated from a traditional telecommunication fiber can be integrated into conventional cell culture equipment and used for real-time and label-free monitoring of cellular responses to chemical stimuli. The sensing mechanism used for the measurement of cellular responses is based on the excitation of surface plasmon resonance (SPR) on the surface of the optical fiber. In this proof of concept study, the sensor was utilized to investigate the influence of a number of different stimuli on cells-we tested the effects of trypsin, serum and sodium azide. These stimuli induced detachment of cells from the sensor surface, uptake of serum and inhibition of cellular metabolism, accordingly. The effects of different stimuli were confirmed with alamar blue assay, phase contrast and fluorescence microscopy. The results indicated that the fiber biosensor can be successfully utilized for real-time and label-free monitoring of cellular response in the first 30 min following the introduction of a stimulus. Furthermore, we demonstrated that the optical fiber biosensors can be easily regenerated for repeated use, proving this platform as a versatile and cost-effective sensing tool. |Animals [MESH] |Cattle [MESH] |Cell Culture Techniques/*instrumentation [MESH] |Equipment Design [MESH] |Mice [MESH] |NIH 3T3 Cells [MESH] |Optical Fibers [MESH] |Serum/metabolism [MESH] |Sodium Azide/metabolism [MESH] |Surface Plasmon Resonance/*instrumentation [MESH]Surface plasmon resonance fiber sensor for real-time and label-free mo(epmc).pdf DeepDyve Pubget Overpricing