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10.1117/1.JBO.19.4.045001

http://scihub22266oqcxt.onion/10.1117/1.JBO.19.4.045001
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C3974550!3974550!24699632
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suck abstract from ncbi


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pmid24699632      J+Biomed+Opt 2014 ; 19 (4): ä
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  • Four-dimensional motility tracking of biological cells by digital holographic microscopy #MMPMID24699632
  • Yu X; Hong J; Liu C; Cross M; Haynie DT; Kim MK
  • J Biomed Opt 2014[Apr]; 19 (4): ä PMID24699632show ga
  • Three-dimensional profiling and tracking by digital holography microscopy (DHM) provide label-free and quantitative analysis of the characteristics and dynamic processes of objects, since DHM can record real-time data for microscale objects and produce a single hologram containing all the information about their three-dimensional structures. Here, we have utilized DHM to visualize suspended microspheres and microfibers in three dimensions, and record the four-dimensional trajectories of free-swimming cells in the absence of mechanical focus adjustment. The displacement of microfibers due to interactions with cells in three spatial dimensions has been measured as a function of time at subsecond and micrometer levels in a direct and straightforward manner. It has thus been shown that DHM is a highly efficient and versatile means for quantitative tracking and analysis of cell motility.
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