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10.1111/cmi.12233 http://scihub22266oqcxt.onion/10.1111/cmi.12233 C3969633!3969633 !24164701     free     free     free Deprecated: Implicit conversion from float 233.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 233.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 233.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 233.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 233.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 233.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 233.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 267.2 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 267.2 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 267.2 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Warning: imagejpeg(C:\Inetpub\vhosts\kidney.de\httpdocs\phplern\24164701 .jpg): Failed to open stream: No such file or directory in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 117       Cell+Microbiol 2014 ; 16 (4 ): 451-65 Nephropedia Template TP {{tp|p=24164701 |t=2014. Cytoplasmic replication of Staphylococcus aureus upon phagosomal escape triggered by phenol-soluble modulin ? |pdf=|usr=}}{{24164701 }} gab.com Text Cytoplasmic replication of Staphylococcus aureus upon phagosomal escape triggered by phenol-soluble modulin JO: Cell Microbiol http://www.kidney.de/pget.php?&tw=1&pmid=24164701 #FOAvip Staphylococcus aureus is a Gram-positive human pathogen that is readily internalized by professional phagocytes such as macrophages and neutrophils but also by non-professional phagocytes such as epithelial or endothelial cells. Intracellular bacteria have been proposed to play a role in evasion of the innate immune system and may also lead to dissemination within migrating phagocytes. Further, S.?aureus efficiently lyses host cells with a battery of cytolytic toxins. Recently, phenol-soluble modulins (PSM) have been identified to comprise a genus-specific family of cytolytic peptides. Of these the PSM? peptides have been implicated in killing polymorphonuclear leucocytes after phagocytosis. We questioned if the peptides were active in destroying endosomal membranes to avoid lysosomal killing of the pathogen and monitored integrity of infected host cell endosomes by measuring the acidity of the intracellular bacterial microenvironment via flow cytometry and by a reporter recruitment technique. Isogenic mutants of the methicillin-resistant S.?aureus (MRSA) strains USA300 LAC, USA400 MW2 as well as the strongly cytolytic methicillin-sensitive strain 6850 were compared with their respective wild type strains. In all three genetic backgrounds, PSM? mutants were unable to escape from phagosomes in non-professional (293, HeLa, EAhy.926) and professional phagocytes (THP-1), whereas mutants in PSM? and ?-toxin as well as ?-toxin, phosphatidyl inositol-dependent phospholipase C and Panton Valentine leucotoxin escaped with efficiencies of the parental strains. S.?aureus replicated intracellularly only in presence of a functional PSM? operon thereby illustrating that bacteria grow in the host cell cytoplasm upon phagosomal escape. Twit Text FOAVip Cytoplasmic replication of Staphylococcus aureus upon phagosomal escape triggered by phenol-soluble modulin ????Cell Microbiol http://www.kidney.de/pget.php?&tw=1&pmid=24164701 #FOAvip Twit Text # Free Paper on # # # # # LINK http://www.kidney.de/pget.php?&tw=1&pmid=24164701 #FOAvip English Wikipedia <ref>{{Cite pmid|24164701 }}</ref> Cytoplasmic replication of Staphylococcus aureus upon phagosomal escape triggered by phenol-soluble modulin ? #MMPMID24164701 Grosz M ; Kolter J ; Paprotka K ; Winkler AC ; Schäfer D ; Chatterjee SS ; Geiger T ; Wolz C ; Ohlsen K ; Otto M ; Rudel T ; Sinha B ; Fraunholz M Cell Microbiol 2014[Apr]; 16 (4 ): 451-65 PMID24164701 show ga Staphylococcus aureus is a Gram-positive human pathogen that is readily internalized by professional phagocytes such as macrophages and neutrophils but also by non-professional phagocytes such as epithelial or endothelial cells. Intracellular bacteria have been proposed to play a role in evasion of the innate immune system and may also lead to dissemination within migrating phagocytes. Further, S.?aureus efficiently lyses host cells with a battery of cytolytic toxins. Recently, phenol-soluble modulins (PSM) have been identified to comprise a genus-specific family of cytolytic peptides. Of these the PSM? peptides have been implicated in killing polymorphonuclear leucocytes after phagocytosis. We questioned if the peptides were active in destroying endosomal membranes to avoid lysosomal killing of the pathogen and monitored integrity of infected host cell endosomes by measuring the acidity of the intracellular bacterial microenvironment via flow cytometry and by a reporter recruitment technique. Isogenic mutants of the methicillin-resistant S.?aureus (MRSA) strains USA300 LAC, USA400 MW2 as well as the strongly cytolytic methicillin-sensitive strain 6850 were compared with their respective wild type strains. In all three genetic backgrounds, PSM? mutants were unable to escape from phagosomes in non-professional (293, HeLa, EAhy.926) and professional phagocytes (THP-1), whereas mutants in PSM? and ?-toxin as well as ?-toxin, phosphatidyl inositol-dependent phospholipase C and Panton Valentine leucotoxin escaped with efficiencies of the parental strains. S.?aureus replicated intracellularly only in presence of a functional PSM? operon thereby illustrating that bacteria grow in the host cell cytoplasm upon phagosomal escape. |Bacterial Toxins/*metabolism [MESH] |Carboxylic Acids/*analysis [MESH] |Cell Line [MESH] |Cytoplasm/*microbiology [MESH] |Epithelial Cells/microbiology [MESH] |Fibroblasts/microbiology [MESH] |Flow Cytometry [MESH] |Humans [MESH] |Monocytes/microbiology [MESH] |Phagosomes/*chemistry/*drug effects/microbiology [MESH]Cytoplasmic replication of Staphylococcus aureus upon phagosomal escap(epmc).pdf DeepDyve Pubget Overpricing