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10.1007/s10815-013-0164-1

http://scihub22266oqcxt.onion/10.1007/s10815-013-0164-1
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C3969462!3969462!24390627
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suck abstract from ncbi


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pmid24390627      J+Assist+Reprod+Genet 2014 ; 31 (4): 447-51
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  • Vitrification of human blastocysts previously cryopreserved by slow controlled-rate freezing at the cleavage stage #MMPMID24390627
  • Lierman S; Van den Abbeel E; De Sutter P
  • J Assist Reprod Genet 2014[Apr]; 31 (4): 447-51 PMID24390627show ga
  • Purpose: The objective of this study was to evaluate the efficiency of vitrified blastocysts derived from frozen-thawed cleavage stage embryos in terms of morphological survival and re-expansion status of the blastocoelic cavity. Results: After warming 162 blastocysts derived from fresh embryos (= control group) and 90 blastocysts from frozen-thawed cleavage stage embryos (= study group) and after 2?3 h of in vitro culture the percentage of blastocysts with morphological survival was not different between the two groups. After 24 h of in vitro culture, the percentage of fully expanded, hatching or hatched blastocysts was not different between both groups. Conclusion(s): The results show that blastocysts derived from frozen-thawed cleavage stage embryos can be cryopreserved successfully a second time by vitrification method. Re-cryopreservation by vitrification still needs to be approached with some caution because little data on long term safety of multiple freezing is available.
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