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10.1681/ASN.2013020178 http://scihub22266oqcxt.onion/10.1681/ASN.2013020178 C3968489!3968489!24309184     free     free     free Deprecated: Implicit conversion from float 233.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 233.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 233.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 233.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534       J+Am+Soc+Nephrol 2014 ; 25 (4): 681-6 Nephropedia Template TP {{tp|p=24309184|t=2014. Two-Photon Microscopy Reveals Stationary Podocytes in Living Zebrafish Larvae |pdf=|usr=}}{{24309184}} gab.com Text Two-Photon Microscopy Reveals Stationary Podocytes in Living Zebrafish Larvae JO: J Am Soc Nephrol http://www.kidney.de/pget.php?&tw=1&pmid=24309184 #FOAvip Podocytes are an essential component of the glomerular filtration barrier and cover the outer aspect of glomerular capillaries. They form a complex actin-based cytoskeleton in vivo and show prominent motility in vitro, but whether podocytes are stationary or mobile in vivo is debated. To address this question, the pronephros of translucent zebrafish larvae (casper) expressing enhanced green fluorescent protein (eGFP) specifically in podocytes (wt1a:eGFP larvae) was observed by intravital two-photon microscopy over extended periods of time. Podocyte cell bodies and the interdigitating branching pattern of major processes could be resolved with a resolution of approximately 1 µm in the xy-plane. Time-lapse imaging of zebrafish larvae at 5?7 days after fertilization demonstrated that podocytes neither migrated nor changed the branching pattern of their major processes over a time period of up to 23 hours. In summary, we show by extended intravital two-photon microscopy that podocytes are stationary cells in the intact glomerulus of a translucent zebrafish with fluorescently-labeled podocytes. Twit Text FOAVip Two-Photon Microscopy Reveals Stationary Podocytes in Living Zebrafish Larvae ????J Am Soc Nephrol http://www.kidney.de/pget.php?&tw=1&pmid=24309184 #FOAvip Twit Text # Free Paper on # # # # # LINK http://www.kidney.de/pget.php?&tw=1&pmid=24309184 #FOAvip English Wikipedia <ref>{{Cite pmid|24309184}}</ref> Two-Photon Microscopy Reveals Stationary Podocytes in Living Zebrafish Larvae #MMPMID24309184 Endlich N; Simon O; Göpferich A; Wegner H; Moeller MJ; Rumpel E; Kotb AM; Endlich K J Am Soc Nephrol 2014[Apr]; 25 (4): 681-6 PMID24309184show ga Podocytes are an essential component of the glomerular filtration barrier and cover the outer aspect of glomerular capillaries. They form a complex actin-based cytoskeleton in vivo and show prominent motility in vitro, but whether podocytes are stationary or mobile in vivo is debated. To address this question, the pronephros of translucent zebrafish larvae (casper) expressing enhanced green fluorescent protein (eGFP) specifically in podocytes (wt1a:eGFP larvae) was observed by intravital two-photon microscopy over extended periods of time. Podocyte cell bodies and the interdigitating branching pattern of major processes could be resolved with a resolution of approximately 1 µm in the xy-plane. Time-lapse imaging of zebrafish larvae at 5?7 days after fertilization demonstrated that podocytes neither migrated nor changed the branching pattern of their major processes over a time period of up to 23 hours. In summary, we show by extended intravital two-photon microscopy that podocytes are stationary cells in the intact glomerulus of a translucent zebrafish with fluorescently-labeled podocytes. äTwo-Photon Microscopy Reveals Stationary Podocytes in Living Zebrafish(epmc).pdf DeepDyve Pubget Overpricing