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Testing the anti-fibrotic potential of the single-chain Fv antibody against the
?2 C-terminal telopeptide of collagen I
#MMPMID24195607
Fertala J
; Kostas J
; Hou C
; Steplewski A
; Beredjiklian P
; Abboud J
; Arnold WV
; Williams G
; Fertala A
Connect Tissue Res
2014[Apr]; 55
(2
): 115-22
PMID24195607
show ga
Abstract This study focuses on the single-chain fragment variable (scFv) variant
of the original IgA-type antibody, recognizing the ?2 C-terminal telopeptide
(?2Ct) of human collagen I, designed to inhibit post-traumatic localized fibrosis
via blocking the formation of collagen-rich deposits. We have demonstrated that
the scFv construct expressed in yeast cells was able to fold into an
immunoglobulin-like conformation, but it was prone to forming soluble aggregates.
Functional assays, however, indicate that the scFv construct specifically binds
to the ?2Ct epitope and inhibits collagen fibril formation both in vitro and in a
cell culture model representing tissues that undergo post-traumatic fibrosis.
Thus, the presented study demonstrates the potential of the scFv variant to serve
as an inhibitor of the excessive formation of collagen-rich fibrotic deposits,
and it reveals certain limitations associated with the current stage of
development of this antibody construct.
|Cell Line
[MESH]
|Cicatrix/drug therapy/genetics/immunology
[MESH]
|Collagen Type I/*chemistry/genetics/immunology
[MESH]
free
free
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